腰椎退变黄韧带中炎性细胞因子的表达

目的:观察腰椎退变黄韧带中炎性细胞因子的表达情况,探讨其在腰椎黄韧带退变过程中的作用。方法:收集腰椎退变性疾病患者在后路椎板切除减压治疗时取下的黄韧带标本40例(退变组),术前通过CT、MRI证实均有黄韧带肥厚;以10例脊柱外伤行后路手术治疗的青年患者的腰椎黄韧带作为对照(对照组)。将术中切除的黄韧带剔除骨、脂肪及筋膜等组织,修成约2cm×1.5cm×0.3cm,在10%福尔马林溶液中固定24～48h后取出,制备成石蜡切片,采用苏木精伊红(HE)染色,应用光学显微镜观察病理学改变,并用免疫组化技术观察黄韧带标本中白介素-1(IL-1)、IL-6和肿瘤坏死因子-ɑ(TNF-ɑ)的表达情况。结果:对照组黄韧带弹力纤维呈波浪状,排列规则,弹力纤维间可见少量的胶原纤维和散在的成纤维细胞(22.56±6.05个/HP);退变组黄韧带弹力纤维明显减少,胶原纤维增多,并且伴软骨细胞、成纤维细胞、毛细血管的增生,成纤维细胞计数为62.66±18.40个/HP,两组成纤维细胞计数差异有统计学意义(P<0.05)。退变黄韧带组织中IL-1、IL-6、TNF-α表达阳性率分别为75%、77.5%、82.5%,对照组黄韧带组织中的表达阳性率分别为40%、20%、40%,退变组明显高于对照组(P<0.05)。结论:IL-1、IL-6、TNF-ɑ在腰椎退变黄韧带中有较高的表达,这些炎性因子可能参与了腰椎黄韧带退变的过程。

The expression of inflammatory cytokines in ligamentum flavum of the degenerative lumbar and its clinical significance

Objective:To investigate the expression and effect of inflammatory cytokine in ligamentum flavum and the mechanism in degeneration.Method:40 ligamentum flavum specimens were harvested from 40 patients(degenerative group).All cases presented with ligamentum flavum hypertrophy on CT and MRI imaging and all underwent decompressive laminectomy.Specimens from 10 cases with spine trauma and underwent surgery were regarded as normal controls(normal group).The ligamentum flavum were free of bone,fat and soft tissue...