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| 低剂量铅对原代培养的海马神经元的影响 | |
| 引用本文： | 胡前胜,董胜璋,陈学敏,傅洪军,任铁玲. 低剂量铅对原代培养的海马神经元的影响[J]. 中国公共卫生, 2003, 19(5): 518-520 |
| 作者姓名： | 胡前胜 董胜璋 陈学敏 傅洪军 任铁玲 |
| 作者单位： | 1. 中山大学公共卫生学院,广州,510080;华中科技大学同济医学院公共卫生学院 2. 中山大学公共卫生学院,广州,510080 3. 华中科技大学同济医学院公共卫生学院 |
| 基金项目： | 国家自然科学基金资助 (30 2 71 1 0 9) |
| 摘 要： | 目的 研究低剂量铅对原代培养的海马神经元生长及存活的影响。方法 建立新生大鼠海马神经元原代无血清培养技术，通过不同浓度PbCl2染毒后，于不同时间点观察神经细胞的生长和存活情况。结果 极低浓度的铅(10^-7mol／L)即可引起神经元出芽延迟，突起长度缩短，细胞存活率下降，随剂量增加和时间延长，毒效应越严重。结论 低剂量铅具有抑制神经元生长和存活的毒性。 |
| 关 键 词： | 铅 海马 神经元 神经突起 |
| 文章编号： | 1001-0580(2003)05-0518-03 |
| 修稿时间： | 2002-12-02 |
Impact of low-level lead on growth and survival of primary cultured hippocampus neurons |
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| HU Qian-sheng,DONG Sheng-zhang,CHEN Xue-min,et al.. Impact of low-level lead on growth and survival of primary cultured hippocampus neurons[J]. Chinese Journal of Public Health, 2003, 19(5): 518-520 | |
| Authors: | HU Qian-sheng DONG Sheng-zhang CHEN Xue-min et al. |
| Affiliation: | HU Qian-sheng,DONG Sheng-zhang,CHEN Xue-min,et al.School of Public Health Sun Yat-sen University |
| Abstract: | Objective To study the impact of low-level lead on the growth and survival of primary cultured hippocampus neurons.Methods To establish the technique of primary culturing hippocampus neurons of newborn rat with serum-free medium.Being treated with different dosage of PbCl 2,the growth and survival of the cultured cells were observed at different time-point.Results Even low level of 10 -7 mol/L Pb 2+ could delay the neurite's outgrowth,shorten the neurite's length and decrease the neuron's survival rate.When Pb 2+ increased,its toxic effects increased.Conclusion Low-level lead inhibited the neurons' growth and survival. |
| Keywords: | lead hippocampus neuron neurite |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |