基于关键基因与核心microRNA探讨大黄-黄连药对抗Hp感染的分子机制

目的:基于生物信息学和网络药理学探究大黄-黄连药对(RRRCR)治疗Hp感染胃肠道疾病的分子机制,阐明其靶向作用的核心基因及关键microRNA.方法:通过TCMSP数据库筛选RRRCR的活性成分及作用靶点;通过GEO数据库检索Hp感染胃黏膜上皮的基因表达谱芯片数据;通过R软件对芯片数据进行均一化处理并分析差异表达基因...

To investigate the molecular mechanism underlying Rhei Radix et Rhizoma and Coptidis Rhizoma herb pair against helicobacter pylori infection based on core genes and key micrornas

Objective:To investigate the molecular mechanism of Rhei Radix et Rhizoma and Coptidis Rhizoma(RRRCR)in the treatment of helicobacter pylori(Hp)related gastrointestinal diseases based on bioinformatics and network pharmacology,and to elucidate the core genes and key microRNAs targeted by RRRCR.Methods:TCMSP database was used to screen the active components and targets of RRRCR.GEO database was used to search the gene expression profile of Hp infected gastric mucosa.R software was used to homogenize chip data and analyze differentially expressed genes.STRING database was used to construct PPI network of component targets and disease targets and to extract the intersection of these two networks.DAVID database was used for enrichment analysis of GO and KEGG of the intersection genes.The core genes were screened by CytoHubba and the key microRNAs were analyzed by TargetScan.Results:Twenty-one RRRCR active components and 217 targets were obtained.The data of GSE70394 chip included 128 differentially expressed genes.Further analysis showed that there were 55 specific gene targets for RRRCR in the treatment of Hp related gastrointestinal diseases,which were mainly involved in the biological processes such as immune and inflammatory reactions,and their molecular functions were mainly reflected in TNF activated receptors,cytokine receptors,and mainly enriched in extracellular space,endoplasmic reticulum membrane and other areas.The mechanism of RRRCR against Hp infection mainly involves JAK-STAT signaling pathway,NF-κB signaling pathway,etc.The ten core genes of RRRCR in the treatment of Hp related gastrointestinal diseases include CXCL8,IL-10,IL-1β,etc,and the ten key microRNAs include microRNA-122-5 p,microRNA-93-5 p,microRNA-558,etc.Conclusion:RRRCR plays a significant role in the treatment of Hp related gastrointestinal diseases by regulating the coding RNA(gene)and non-coding RNA(microRNA),showing the characteristics of multi-component,multi-target,and multi-channel effects.