不同浓度牡蛎蛋白肽对大鼠骨髓间充质干细胞增殖和分化能力的影响

目的:探讨不同浓度牡蛎蛋白肽对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的影响.方法:选择具备稳定传代能力的第3代大鼠BMSCs,采用MTT试剂盒,检测细胞在0、10、20、50 mg/L不同牡蛎蛋白肽浓度干预培养24 h、3 d、7 d后的细胞活力.然...

Effects of oyster protein peptide with different concentrations on the proliferation and differentiation of bone marrow mesenchymal stem cells

PURPOSE: To investigate the effect of different concentrations of oyster protein peptide on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs). METHODS: BMSCs of the third generation from rats with stable subculture ability were selected, and the cell viability was detected by MTT kit at different concentrations of 0,10, 20 and 50 mg/L oyster protein peptide for 24 hours, 3 days and 7 days. BMSCs were divided into two groups: cultured with DMEM or ODM. According to the different concentrations of oyster protein peptide, the experimental groups were divided into four subgroups, which were 0, 10, 20 and 50 mg/L, respectively. The effects of different concentrations of oyster protein peptide on the early osteogenic differentiation of BMSCs were evaluated by ALP staining and ALP semiquantitative detection. Alizarin red staining was used to detect the effect of different concentrations of oyster protein peptide on the osteogenesis and mineralization of BMSCs after 21 days of treatment. RT-PCR was used to defect the expression of bone formation-related genes. SPSS 20.0 software package was used to analyze the data. RESULTS: The results of MTT showed that cell activity was vigorous and there was no obvious abnormality after intervention with different concentrations of drugs, but the number of cell death increased when the concentration of oyster protein peptide was 50 mg/L. The results of ALP semi-quantitative analysis showed that ALP activity of DMEM and ODM group was significantly higher than that of the control group(P<0.05) on the 10 th day of differentiation induction, but there was no significant difference between 50 mg/L group and the control group. Alizarin red semi-quantitative analysis showed that the A value of 20 mg/L in ODM group was significantly higher than that of the control group(P<0.05). The expression of RUNX-2 and OCN in 10 mg/L and 20 mg/L groups were significantly higher than that in control goup and 50 mg/L group(P<0.05).CONCLUSIONS: Oyster protein peptide with a concentration higher than 50 mg/L can significantly inhibit osteogenic differentiation of BMSCs, while oyster protein peptide with a low concentration, especially oyster protein peptide with a concentration of 20 mg/L, can promote osteogenic differentiation and cell growth of BMSCs.