miR-338-3p靶向PTP1B抑制胃癌细胞迁移和侵袭

目的:探讨miR-338-3p和PTP1B对胃癌细胞迁移和侵袭的影响.方法:Western Blot法测定PTP1B在胃癌标本及癌旁组织中的表达.通过qRT-PCR检测miR-338-3p的表达.用PTP1B质粒和siRNA、miR-338-3p类似物转染SGC7901细胞,用Transwell法测定miR-338-3...

miR-338-3p inhibits cell migration and invasion in gastric cancer cells by targeting PTP1B

Objective:To investigate the effects of miR-338-3p and PTP1B on the migration and invasion of gastric cancer cells.Methods:Western Blot was used to test the expression of PTP1B in gastric cancer specimens and adjacent tissues.qRT-PCR was used to detect the expression of miR-338-3p.SGC7901 cells were transfected by PTP1B plasmid and siRNA,miR-338-3p mimics.Transwell assay was used to detect cell migration and invasion.Finally,we verified the targeting of PTP1B by miR-338-3p by the dual luciferase reporter gene.Results:In this study,we found that PTP1B protein level was obviously increased in gastric cancer tissues and that PTP1B promoted the migration and invasion of gastric cancer cell lines.Bioinformatics analysis identified that,miR-338-3p might potentially target PTP1B.In gastric cancer tissue,miR-338-3p was negatively correlated with PTP1B(r=-0.653,P=0.013).We demonstrated that miR-338-3p regulated PTP1B in gastric cancer cells and that it regulated the migration and invasion of gastric cancer cells.Conclusion:miR-338-3p can inhibit the migration and invasion of gastric cancer cells,which may be related to targeting PTP1B,which may provide new insights into novel molecular therapeutic targets for gastric cancer.