脂联素、瘦素、抵抗素的表达对判断结直肠癌临床分期及预后的价值

目的:探讨结直肠癌组织中脂联素、瘦素、抵抗素表达水平与结直肠癌临床分期的关系及对预后判断的价值.方法:选取2015年2月-2016年2月收治的结直肠癌患者56例作为研究对象,术中收集癌组织和癌旁组织,另选取同期收治的行纤维结肠镜活检或切除并经病理证实的结直肠腺瘤患者52例为结直肠腺瘤组.分别采用免疫组织化学染色法和实时...

Value of adiponectin,leptin and resistin expression in judging the clinical stage and prognosis of colorectal cancer

Objective:To investigate the relationships between the expressions of adiponectin,leptin,resistin in colorectal cancer tissues and the clinical stage of colorectal cancer and its prognostic value.Methods:56 patients with colorectal cancer admitted from February 2015 to February 2016 were selected as the study objects,the cancer tissues and adjacent tissues were collected during the operation,and 52 patients with colorectal adenoma who underwent colonoscopy biopsy or resection and confirmed by pathology were selected as the colorectal adenoma group.The expression levels of adiponectin,leptin,resistin proteins and mRNAs were detected by immunohistochemistry and real-time fluorescence quantitative analysis(qRT-PCR),and the relationships between the three levels and the clinicopathological characteristics,prognosis of patients were analyzed.Results:The expression levels of adiponectin protein and mRNA in paracancerous tissue,colorectal adenoma tissue and colorectal cancer tissue were significantly decreased(P<0.05),and leptin,resistin protein and mRNA expression levels were significantly increased(P<0.05).The proportion of TNM stageⅢ-Ⅳ,Dukes stage C+D,lymph node metastasis and death rate in low adiponectin expression,leptin high expression and resistin high expression group were significantly higher than those in high adiponectin expression group,low leptin expression group and low resistin expression group(P<0.05).TNM stageⅢ-Ⅳ,Dukes stage C+D,lymph node metastasis,low level of adiponectin,high level of leptin and high level of resistin were independent risk factors for poor prognosis in patients with colorectal cancer(P<0.05).The area under the curve of predicting the adverse prognosis of patients by the combined detection of the three was 0.926(95%CI:0.855~0.996),the sensitivity was 90.60%,and the specificity was 87.50%.Conclusion:The expression level of adiponectin in colorectal cancer tissue is significantly decreased,the expression levels of leptin and resistin are significantly increased,which may be related to the increases of clinical stage and adverse prognosis risk.The combination of the three may be used as biomarkers to evaluate the prognosis of colorectal cancer patients.