IL-37对变应性鼻炎患者相关细胞因子免疫调控研究

目的 探究白介素37(1L-37)在变应性鼻炎(AR)患者外周血单核细胞(PBMCs)的表达水平及对相关细胞因子的免疫调控分析.方法 选取120例AR患者作为研究组,另选择同期健康志愿者120例作为对照组,检测各组血清IL-37蛋白及mRNA表达,辅助性T细胞1(Th1)、Th2、Th17、CD4+CD25+调节性T细...

Study on the immunoregulation of IL-37 on related cytokines in patients with allergic rhinitis

Objective To explore the expression level of interleukin 37(IL-37)in peripheral blood mononuclear cells(PBMCs)of patients with allergic rhinitis(AR)and analyze the immunoregulation of related cytokines.Methods 120 patients with AR were selected as the research group,and another 120 healthy volunteers during the same period were selected as the control group.Serum IL-37 protein and mRNA expression,helper T cell 1(Th1),Th2,Th17,CD4+CD25+regulatory T cells(Treg cells)and Foxp3+CD4+CD25+Treg cell percentages and related cytokine levels were detected in each group.The recombinant human IL-37 protein(rIL-37)at concentrations of 100 ng/mL and 200 ng/mL was used to intervene to analyze the changes in related cytokine levels before and after the intervention.Results The serum IL-37 level and IL-37 mRNA expression in the study group were significantly lower than those in the control group.The percentages of Th1 cells and Th17 cells and Th1/Th2 ratio were significantly higher than those in the control group.Th2 cells,CD4+CD25+Treg cells,Foxp3+The percentage of CD4+CD25+Treg cells was significantly lower than that of the control group(P<0.05).The levels of IL-1β,IL-6,and IL-17 in the study group were significantly higher than those in the control group,and the levels of IL-4,IL-10,IL-27,IFN-γ,and TGF-β1 were significantly lower than those in the control group(P<0.05).Serum IL-37 is negatively correlated with IL-1β,IL-6,IL-17,and positively correlated with IL-4,IL-10,IL-27,IFN-γ,and TGF-β1(P<0.05).After the intervention of rIL-37,the indicators of IL-1β,IL-4,IL-6,IL-10,IL-17,IL-27,IFN-γ,and TGF-β1 in the study group changed more significantly,and the difference was statistically significant(P<0.05).Compared with the 100 ng/mL group,the changes in the levels of cytokines in the intervention PBMCs of the 200 ng/mL group were more significant(P<0.05).Conclusion The expression of IL-37 in patients with allergic rhinitis is significantly decreased,which affects the balance of Th1/Th2,Th17/Treg cells,resulting in a decrease in the level of anti-inflammatory factors and an increase in the level of pro-inflammatory factors,which play an important role in the pathogenesis of AR.