玄参中环烯醚萜甙和苯丙素甙对LTB4产生及血小板聚集的影响

目的：探讨玄参化学成分和药理活性关系。方法：应用大孔树脂、葡聚糖凝胶和正、反相硅胶等层析方法,摸索多种溶剂系统对玄参中含量高的水溶性成分进行分离,应用化学和光谱方法鉴定结构。观察分离得到的主要单体成分对大鼠腹腔中性白细胞中花生四烯酸(AA)代谢产物白三烯B4(LTB4)的影响,及对阈剂量的二磷酸腺苷,AA和20nmol/LPAF诱导的血小板聚集的抑制作用。结果：共分得11种玄参水溶性成分,发现它们属于环烯醚萜甙和苯丙素甙类型。药理实验中苯丙素甙XS-8,XS-10在0.5mmol/L时对LTB4产生较强抑制作用;环烯醚萜甙XS-6,XS-7在相同条件下作用较弱。苯丙素甙和环烯醚萜甙在0.5mmol/L时,对体外诱导的血小板聚集都有不同程度的作用,但苯丙素甙的作用强于环烯醚萜甙。结论：玄参中的苯丙素甙和环烯醚萜甙成分与玄参的抗炎和抗血小板聚集作用有关。

Iridoid and phenylpropanoid glycosides of Scrophularia ningpoensis inhibit the formation of LTB4 and platelet aggregation

Objective: To investigate the relationship between constituents and pharmacological effects of Scrophularia ningpoensis (XuanSheng). Methods: The isolation and identification of compounds were done mainly by no n-polar macroreticular resin, sephadex LH-20, ODS, silica gel column chromatography and FABMS, NMR etc, spectra. Results: The inhibition of the LTB 4 f ormation in the rat abdominal neutral leukocytes by drugs isolated from S. nin gpoensis were observed. The iridoid glycosides XS-6, XS-7 showed weak action , but the phenylpropanoid glycosides XS-8, XS-10 showed potent action at the c oncentration of 0.5 mmol/L. In the inhibition of the rabbit platelet aggregation, iridoid and phenylpropanoid glycosides were active in inhibiting the rabbit threshold ADP-induced and AA-induced but not 20 nmol/L PAF-induced pl atelet aggregation at the concentration of 0.5 mmol/L in vitro. Conclusion: The phenylpropanoid and iridoid glycosides may be associated with anti-inflammation and anti-platelet aggregation effects of S. ningpoensis.