| E3泛素连接酶HECTD3真核表达质粒的构建 |
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| 引用本文： | 卢敏莹,杨波. E3泛素连接酶HECTD3真核表达质粒的构建[J]. 中国实用医药, 2013, 0(33): 253-254 |
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| 作者姓名： | 卢敏莹 杨波 |
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| 作者单位： | 广州医科大学附属肿瘤医院肿瘤研究所,510095 |
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| 摘 要： | 目的：克隆E3泛素连接酶（homologous to the E6-associated protein carboxyl terminus domain containing 3, HECTD3）基因及构建真核表达质粒。方法提取人卵巢癌细胞SKOV-3细胞RNA,并逆转录为cDNA,以此作为模板PCR法扩增HECTD3基因,将其克隆至真核表达载体pcDNA3.1（＋）,测序验证构建质粒中包含HECTD3基因。结论 HECTD3基因克隆及真核表达质粒构建成功,可以用于后续卵巢癌发生发展的分子生物学研究。
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| 关 键 词： | E3泛素连接酶 卵巢癌 真核表达质粒 |
Construction of eukaryotic expression plasmid containing HETCD3 gene |
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| LU Min-ying,YANG Bo. Construction of eukaryotic expression plasmid containing HETCD3 gene[J]. China Practical Medical, 2013, 0(33): 253-254 |
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| Authors: | LU Min-ying YANG Bo |
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| Affiliation: | , HE Zhi-min Cancer Research Center, Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China |
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| Abstract: | Objective To clone the gene of homologous to the E6-associated protein carboxyl terminus domain containing 3 （HECTD3） and construct the HECTD3 gene expression plasmid. Methods The HECTD3 gene was cloned from ovarian cell cDNA by PCR and recombined into the eukaryotic expression plasmid pcDNA3.1（＋）. Results the HECTD3 gene was confirmed in the eukaryotic expression plasmid by sequence analysis. Conclusion The HECTD3 gene expression plasmid can be the tools for further research of ovarian cancer. |
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| Keywords: | Homologous to the E6-associated protein carboxyl terminus domain Containing 3（HETCD3） Ovarian cancer Eukaryotic expression plasmid |
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