X线照射原代培养大鼠海马神经元后p35、p25的表达及Cdk5激酶活性变化

目的 研究X线照射培养大鼠海马神经元后p35,p25的表达及Cdk5激酶活性变化,为放射性脑损伤的预防和治疗提供理论依据.方法 30 Gy X射线单次照射培养至12 d的海马神经元,用Western-blotting检测Cdk5的激活蛋白p35、p25的水平,用DAPI染核方法 检测海马神经元凋亡情况.结果 p35蛋白水平在照射后3.5和4 h分别比对照组升高(1.51±0.13)、(1.45±0.14)倍,差异均有统计学意义(P<0.01);p25蛋白水平在照射后6 h比对照组升高(1.62±0.28)倍,差异有统计学意义(P<0.05).30 Gy组在照射后24 h核固缩百分数为(24.8±3.97)%,与对照组(1.82%±1.08%)有显著性差异(P<0.01);30 Gy+roscovitine组在照射后24 h核固缩百分数为(7.74±2.27)%,与对照组有显著性差异(P<0.01).结论 X线照射培养海马神经元后p35、p25 蛋白表达增加,激活cdk5;应用Cdk5抑制剂roscovitine抑制Cdk5活性可以显著减少神经元的凋亡.

p35 and p25 expressions and Cdk5 kinase activity in primary cultured rat hippocampal neurons with X-ray exposure

Objective To study the expressions of p35 and p25 and Cdk5 kinase activity in cultured rats hippocampal neurons following X-ray exposure to provide experimental evidence for prevention and treatment of radiation encephalopathy.Methods The hippocampal neurons cultured for 12 days were subjected to a single-dose X-ray exposure of 30 Gy.Western blotting was used to detect the p35 and p25 protein levels,and the effect of pretreatment with roscovitine,a Cdk5 inhibitor,on the apoptosis of the hippocampal neurons ...