手针与电针对急性运动大鼠骨骼肌肌浆网Ca~(2+)的影响

目的:研究手针和电针对急性游泳运动大鼠骨骼肌肌浆网Ca2+含量、Ca2+-ATP酶活性的影响,探讨针灸增强运动能力的作用机制。方法:60只SD雄性大鼠随机分为空白对照组、模型组、手针组、电针组。手针组和电针组在急性游泳运动前运用针刺和电针两种不同的方法,对"大椎"命门"环跳"足三里"穴进行20 min刺激。其中手针组运用捻转手法,每间隔3~5 min运针1次(频率约为120~140次/min),每次运针30~60 s;电针组在针刺后接BX型电针仪,电针参数为音频脉冲调制波,频率为500~800 Hz,电流为0.20~0.25 mA。采用比色法分别测定骨骼肌肌浆网Ca2+含量、Ca2+-ATP酶活性指标。结果:骨骼肌肌浆网Ca2+含量模型组明显低于空白对照组、手针组和电针组(P<0.01);肌浆网Ca2+-ATP酶活性模型组亦明显低于空白对照组、手针组和电针组(P<0.05,0.01)。手针组肌浆网Ca2+-ATP酶活性比空白对照组明显升高(P<0.05);手针组Ca2+含量及Ca2+-ATP酶活性与电针组比较,两组间差异无显著性意义(P>0.05)。结论:针刺可以提高大鼠肌浆网Ca2+-ATP酶活性,增强肌浆网对细胞内Ca2+浓度的调节作用,参与肌肉兴奋收缩偶联并维持肌细胞胞质Ca2+稳态,这是针灸增强运动能力的重要因素之一。

Effects of Manual Acupuncture and Electroacupuncture on Ca2+ Content and Ca2+-ATPase Activity in Sarcoplasmic Reticulum of Skeletal Muscle Cells in Rats During Acute Swimming Exercise

OBJECTIVE: To observe the effects of manual acupuncture (MA) and electroacupuncture (EA) on Ca2+ content and Ca2+ -ATPase activity in sarcoplasmic reticulum of skeletal muscle cells in rats of acute swimming exercise (ASE) so as to reveal its underlying mechanism in strengthening motor abilities. METHODS: Sixty male SD rats were randomly divided into control, model, MA and EA groups, with 15 cases in each. Rats in MA group and EA group were treated respectively with MA (120-140 twirlings/min, 30-60 s/time every 3-5 min) and EA (500-800 Hz, 0.20-0.25 mA) stimulation of "Dazhui" (GV 14), "Mingmen" (GV 4), "Huantiao" (GB 30) and "Zusanli" (ST 36) for 20 min before ASE. After ASE (120 min), the rats anesthetized with urethane (1 g/kg) were killed for sampling skeletal muscles (quadriceps muscle of thigh) and for detecting Ca2+ content and Ca2+ -ATPase activity with thymol blue chromatometry and chemical chromatometry respectively. Results In comparison with control group, Ca2+ content and Ca2+ -ATPase activity in sarcoplasmic reticulum of skeletal muscle cells in model group were obviously lower (P < 0.01, 0.05); while compared with model group, both Ca2+ content and Ca2+ -ATPase activity in sarcoplasmic reticulum in MA and EA groups increased significantly (P < 0.01). The activity of Ca2+ -ATPase of MA group was evidently higher than that of control group (P < 0.05). No significant differences were found between MA and EA groups in Ca2+ content and Ca2+ -ATPase activity (P > 0.05). CONCLUSION: Both MA and EA can increase Ca2+ -ATPase activity and Ca2+ content in sarcoplasmic reticulum of skeletal muscle cells in motor fatigue rats, which may contribute to their effects in strengthening motor ability.