肾病综合征鼠骨代谢变化特点及芪归合剂对其的作用

 【目的】 探讨肾病综合征（NS）鼠骨代谢的变化特点及芪归合剂对其的作用。【方法】 制备大鼠阿霉素NS模型，设模型组、激素治疗组、芪归治疗组、激素芪归治疗组及正常对照组，每组8只，用药时间为3周。测量各组尿蛋白、血生化指标和右股骨长度；用HologicQDR-2000＋DEXA行股骨全段骨密度（BMD）测定；以放射免疫法测定血清骨钙素（OC）、Ⅰ型前胶原氨基端伸展肽（PINP）、Ⅰ型胶原羧基端关联肽原（ICTP）水平。【结果】 模型组股骨长度（3．35±0．09）cm，低于正常组（3．53±0．11）cm（P〈0．05）；芪归治疗组（3．46±0．09）cm，高于模型组（P〈0．05）；激素芪归组（3．28±0．07）cm，高于激素治疗组（3．16±0．11）cm（P〈0．01）；激素治疗组低于模型组（P〈0．05）。模型组股骨全段BMD（0．1357±0．0137）g/cm^2，低于正常组（0．1599±0．0101）g／cm^2（P〈0．01）；激素治疗组（0．1208±0．0123）g／cm^2，低于模型组（P〈0．05）。模型组血OC（16．31±3．87）μg，L和PINP（2．02±0．51）μg/L水平，低于正常组，分别（31．49±4．16）μg/L、（3．58±0．46）μg/L（P〈0．01）；芪归治疗组血OC（25．90±8．26）μg/L和PINP（3．42±1．07）μg/L水平，高于模型组（分别P〈0．05和P〈0．01）；激素芪归组血OC（12．01±1．97）μg/L和PINP（2．47±0．88）μg/L水平，高于激素治疗组，分别（6．12±3．26）μg/L、（1．63±0．74）μg/L（P〈0．05）；激素治疗组低于模型组（P〈0．05）。激素治疗组的ICTP（10．15±3．77）μg/L高于模型组（8．71±3．29）μg/L（P〈0．05）。【结论】NS鼠存在骨代谢的异常，激素治疗使骨代谢异常进一步加剧，而芪归合剂治疗对其有改善作用。

Change of Bone Metabolism in Rats with Nephrotic Syndrome and the Role of Astragalus and Angelica Mixture

[Objective] To investigate the change of bone metabolism in rats with nephrotic syndrome(NS)and the role of Astragalus and Angelica mixture(A&A).[Methods] Forty male SD rats(six weeks old)were randomly divided into five groups:control group,NS model group,NS group treated with A&A,NS group treated with dexamethasone(Dex),NS group treated with A&A and Dex.Urine protein,serum biochemical levels and the femur length were measured.The bone mineral density(BMD)of whole part of femur was measured by Hologic ODR-2000+DEXA.Serum osteocalcin(OC),aminoterminal propeptide of type I procollagen(PINP)and carboxyterminal telopeptide of type I collagen(ICTP)levels were assayed by radioimmunoassay technique.[Results] The length of femur of NS group treated with A&A and NS group treated with A&A and Dex were higher than those in NS model group and NS group treated with Dex,respectively(P < 0.05 and P < 0.05).The length of femur of NS group treated with Dex was lower than that of NS model group(P < 0.05).The length of femur of NS model group was lower than that of control group(P < 0.05).The BMD of femur in NS model group was lower than that of control group(P < 0.01).The serum levels of OC and PINP in NS model group were lower than those in control group(P < 0.01).The serum levels of OC and PINP in NS group treated with A&A and NS group treated with A&A and Dex were higher than those in NS model group and NS group treated with Dex,respectively(P < 0.05 and P < 0.01,respectively).The serum levels of OC and PINP in NS group treated with Dex were lower than those in NS model group(P < 0.05).The serum levels of ICTP in NS group treated with Dex were higher than that of NS model group(P < 0.05).[Conclusion] The abnormality of bone metabolism exists in nephrotic rats.Dexamethasone treatment can aggravate this abnormality,which can be improved by Astragalus and Angelica mixture.