Dynamic changes in intranuclear and subcellular localizations of mouse Prrp/DAZAP1 during spermatogenesis: the necessity of the C-terminal proline-rich region for nuclear import and localization |
| |
Authors: | Kurihara Yasuyuki Watanabe Hiroyuki Kawaguchi Atsushi Hori Tamaki Mishiro Kentaro Ono Michio Sawada Hajime Uesugi Seiichi |
| |
Affiliation: | Department of Environment and Natural Sciences, Graduate School of Environment and Information Sciences, Yokohama National University, Hodogaya, Yokohama, Japan. kurihara@mac.bio.bsk.ynu.ac.jp |
| |
Abstract: | Mouse Prrp (mPrrp)/DAZAP1 is a mouse ortholog of Xenopus Prrp, which is involved in vegetal pole localization of Vg1 mRNA in oocytes and is highly expressed in the testis. The mouse protein has been reported to be a shuttling protein which localizes in the nucleus of pre-meiotic spermatogenic cells and round spermatids, and shifts its location into the cytoplasm in elongating spermatids, suggesting that mPrrp may be involved in mRNA transport as well as that of the Xenopus ortholog. We reexamined immunohistochemical analyses of mPrrp/DAZAP1 during spermatogenesis utilizing a newly established monoclonal antibody and reconfirmed it to be a shuttling protein. We also carried out new observations that included remarkable intranuclear movement during spermatogenesis. In addition, we found that a long amino acid stretch which spanned over the C-terminal half of the protein was required for the nuclear import. These observations demonstrated dynamic changes in subnuclear and subcellular localization which might reflect specific functions during spermatogenesis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|