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| Distribution of HBV genotypes among HBV carriers in Benin： phylogenetic analysis and virological characteristics of HBV genotype E | |
| 作者姓名： | Fujiwara K Tanaka Y Orito E Ohno T Kato T Sugihara K Hasegawa I Sakurai M Ito K Ozasa A Sakamoto Y Arita I El-Gohary A Benoit A Ogoundele-Akplogan SI Yoshihara N Ueda R Mizokami M |
| 作者单位： | Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences Nagoya,Japan,Department of Clinical Molecular Informative Medicine Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Clinical Molecular Informative Medicine Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,AIDS Research Center National Institute of Infectious Disease,Tokyo,Japan,Agency for Cooperation in International Health Kumamoto,Japan,Clinical Pathology Department Faculty of Medicine,Suez Canal University,Ismailia,Egypt,Central National Blood Transfusion Center Cotonou,Benin,Hematology Lab-CNHUC Cotonou,Benin,AIDS Research Center National Institute of Infectious Disease,Tokyo,Japan,Department of Internal Medicine and Molecular Science Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan,Department of Clinical Molecular Informative Medicine Nagoya City University Graduate School of Medical Sciences,Nagoya,Japan |
| 摘 要： | AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype. METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing. RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E. The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic diversity (nucleotide homology 96.7-99.2%). Based on the sequences in the basic core promoter (BCP) to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E. CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics, is observed in HBV/E. |
| 关 键 词： | 乙型肝炎病毒 基因型 病毒类型 遗传因素 |
| 收稿时间： | 2005 Feb 15 |
Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E |
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| Fujiwara K,Tanaka Y,Orito E,Ohno T,Kato T,Sugihara K,Hasegawa I,Sakurai M,Ito K,Ozasa A,Sakamoto Y,Arita I,El-Gohary A,Benoit A,Ogoundele-Akplogan SI,Yoshihara N,Ueda R,Mizokami M.Distribution of HBV genotypes among HBV carriers in Benin:phylogenetic analysis and virological characteristics of HBV genotype E[J].World Journal of Gastroenterology,2005,11(41):6410-6415. | |
| Authors: | Fujiwara Kei Tanaka Yasuhito Orito Etsuro Ohno Tomoyoshi Kato Takanobu Sugihara Kanji Hasegawa Izumi Sakurai Mayumi Ito Kiyoaki Ozasa Atsushi Sakamoto Yuko Arita Isao El-Gohary Ahmed Benoit Agossou Ogoundele-Akplogan Sophie I Yoshihara Namiko Ueda Ryuzo Mizokami Masashi |
| Institution: | 1. Department of Internal Medicine and Molecular Science, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan 2. Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya,Japan 3. AIDS Research Center,National Institute of Infectious Disease, Tokyo, Japan 4. Agency for Cooperation in International Health,Kumamoto,Japan 5. Clinical Pathology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt 6. Central National Blood Transfusion Center,Cotonou, Benin 7. Hematology Lab-CNHUC,Cotonou, Benin |
| Abstract: | AIM: To determine the distribution of Hepatitis B virus (HBV) genotypes in Benin, and to clarify the virological characteristics of the dominant genotype. METHODS: Among 500 blood donors in Benin, 21 HBsAg-positive donors were enrolled in the study. HBV genotypes were determined by enzyme immunoassay and restriction fragment length polymorphism. Complete genome sequences were determined by PCR and direct sequencing. RESULTS: HBV genotype E (HBV/E) was detected in 20/21 (95.2%), and HBV/A in 1/21 (4.8%). From the age-specific prevalence of HBeAg to anti-HBe seroconversion (SC) in 19 HBV/E subjects, SC was estimated to occur frequently in late teens in HBV/E. The comparison of four complete HBV/E genomes from HBeAg-positive subjects in this study and five HBV/E sequences recruited from the database revealed that HBV/E was distributed throughout West Africa with very low genetic diversity (nucleotide homology 96.7-99.2%). Based on the sequences in the basic core promoter (BCP) to precore region of the nine HBV/E isolates compared to those of the other genotypes, a nucleotide substitution in the BCP, G1757A, was observed in HBV/E. CONCLUSION: HBV/E is predominant in the Republic of Benin, and SC is estimated to occur in late teens in HBV/E. The specific nucleotide substitution G1757A in BCP, which might influence the virological characteristics, is observed in HBV/E. |
| Keywords: | HBV genotype West Africa Basic core promoter |
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