吲哚美辛肠溶微囊的制备及药物释放的研究

目的用复凝聚法制得吲哚美辛肠溶微囊并探讨其包封率及药物释放情况。方法本实验用复凝聚法制备3种壳聚糖-海藻酸盐吲哚美辛肠溶微囊,并采用紫外分光光度法绘制吲哚美辛在人工肠液(pH 7.4)中的标准曲线。利用紫外分光光度法测定微囊的包封率及测定3种微囊在一定时间内溶出的量作释放研究。结果通过复凝聚法得到吲哚美辛肠溶微囊,粒径1 mm左右,干燥后粒径0.2-0.3 mm,其包封率依次为61.80%,57.00%,64.20%;壳聚糖含量越高,缓释作用越强,微囊中吲哚美辛在人工肠液中的溶出速度明显快于人工胃液。湿微囊溶出高于干微囊。结论紫外分光光度法测定肠溶微囊中吲哚美辛的包封率及释放方法可靠,线性良好,稳定性好;壳聚糖-海藻酸盐微囊使包封药物吲哚美辛有肠溶特性。

The preparation of the indomathacin enteric-coated microcapsules and the investigation of the release of indomathacin

Objective To investigate the entrapment rate and release of indomathacin from enteric-coated microcapsules by the complex coacervation.Methods Three kinds of chitosans-seaweed acid-indomathacin enteric-coated microcapsules were preparated by the complex coacervation in the experiment.The standard curve was made in the artificial intestinal fluid(pH=7.4) with UV spectrophotonetry.The entrapment rate and medicines release of the three kinds microcapsules was measuied by UV spectrophotometry.Results The indomathacin enteric-coated microcapsules was made by coacervation,with a diameter of 1 mm for wet or 0.2-0.3 mm for dry. Its envelopment rate was 61.80%,57.00%,64.20%,respectivdy.The higher the chitosan was,the slower release of indomathacin,in the microcapsules the indomathacin released more amckly in artificial intestinal flnid than in artificial gastric fluid.The wet microcapsules released more quickly than dry microcapsules. Conclusion The method is credibility,the linearity and stability is good.The indomathacin microcapsules entrapped by the chitosans-seaweed showed enteric release specifiaty.