甘肃地区不同民族丙型肝炎患者的HCV基因分型研究

[目的]了解甘肃地区汉族和东乡族丙型肝炎患者的HCV基因分型情况。[方法]对HCV感染的患者样本用实时荧光定量PCR进行病毒载量的确定。对病毒载量大于10。样本用多重PCR进行HCV的基因分型。[结果]通过对甘肃地区汉族、东乡族丙型肝炎感染者的样本进行基因分型，发现两个民族丙型肝炎病毒感染主要为1b型为主，其次是2a型和3a型，但1c型、2c型的感染有显著性差异，汉族以2c型为主，东乡族以1c型为主。[结论]甘肃地区汉族和东乡族丙型肝炎病毒的感染，主要以1b型为主，其次是2a型，和3a型，此外还发现了别的地区少有的1c型和2c型。这两个型不同民族有显著性差异，汉族以2c型为主，东乡族以1C型为主。初步说明甘肃地区丙型肝炎流行的基因型程多样性。

Genotyping of Hepatitis C Virus obtained from patients in Gansu area

[ Objective ] To investigate the genotypes of hepatitis C virus （HCV） in Gansu, and to provide information for the diagnosis and prevention of HCV. [ Methods] Samples obtained from patients with HCV infection were used for viral load determination by real- time quantitative PCR. Samples with viral load greater than 103 were conducted using multiplex PCR for HCV genotyping. [ Results] It was found that lb type was the main infectious virus in Gansu, followed by genotype 2a （21.6%） and 3a （3.2%）. In addition, there was significant difference between lc type （4%） and 2c type （8%）. 2c was dominant genotype in the Han nationality, while lc was dominant in the Dongxiang nationality. [ Conclusion] Genotypes of hepatitis C virus in Gansu are diverse.