| 水翁花对微粒体和神经细胞氧化损伤的保护作用 |
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| 引用本文： | 卢艳花,杜长斌,吴子斌,叶春林,刘建文,魏东芝. 水翁花对微粒体和神经细胞氧化损伤的保护作用[J]. 中国中药杂志, 2003, 28(10): 964-966 |
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| 作者姓名： | 卢艳花 杜长斌 吴子斌 叶春林 刘建文 魏东芝 |
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| 作者单位： | 1. 华东理工大学,生物工程学院,国家重点实验室,上海,200237
2. 香港中文大学,生物化学系 |
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| 摘 要： | 目的 :研究水翁花对小鼠肝微粒体膜脂氧化及H2O2 诱导的PC12神经细胞氧化损伤的保护作用。方法 :用小鼠肝微粒体膜脂氧化模型、细胞培养MTT法测定细胞的存活率等方法。结果 :水翁花水提物能强烈抑制小鼠肝微粒体膜脂氧化 ,IC50为0.013g·L-1,效果优于阳性对照维他命E(IC50为 0.064 g·L-1) ;对H2O2 引起的PC12神经细胞氧化损伤亦显示出较强的抑制作用 ,对神经细胞表现出保护作用。结论 :水翁花对膜脂氧化及神经细胞的氧化损伤的保护作用 ,可能用于治疗与氧化损伤有关的脑疾。
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| 关 键 词： | 水翁花 抗氧化 神经细胞 |
| 文章编号： | 1001-5302(2003)10-0964-03 |
| 收稿时间： | 2002-11-19 |
| 修稿时间： | 2002-11-19 |
Protective Effects of Cleistocalyx operculatus on Lipid Peroxidation and Trauma of Neuronal Cells |
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| LU Yan-hua ;DU Chang-bin ;WU Zi-bin ;YE Chun-lin ;LIU Jian-wen ;WEI Dong-zhi. Protective Effects of Cleistocalyx operculatus on Lipid Peroxidation and Trauma of Neuronal Cells[J]. China Journal of Chinese Materia Medica, 2003, 28(10): 964-966 |
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| Authors: | LU Yan-hua DU Chang-bin WU Zi-bin YE Chun-lin LIU Jian-wen WEI Dong-zhi |
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| Affiliation: | State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China. |
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| Abstract: | OBJECTIVE: To observe the protective effects of Cleistocalyx operculatus on lipid peroxidation in rat liver microsomes and on the trauma of PC12 cells induced by H2O2. METHOD: The mouse liver homogenate lipid peroxidation assay and PC12 Cell culture and Cell viability (MTT assay) were applied. RESULT: Cleistocalyx operculatus showed strong protective effects on lipid peroxidation in rat liver microsomes in a dose-dependent manner and exhibited potent protective effects on the trauma of PC12 cells induced by H2O2 (200 micromol x L(-1)) when the concentration reached 1.00 g x L(-1). CONCLUSION: Cleistocalyx operculatus may be used as antioxidant to prevent or delay the pathogenesis of neural cell diseases. |
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| Keywords: | Cleistocalyx operculatus PC12 cells lipid peroxidation Hydrogen Peroxide |
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