成年兔坐骨神经雪旺细胞的分离纯化培养

目的：探讨从成年新西兰兔坐骨神经分离培养获得大量雪旺细胞的有效手段，方法：利用成年免发生Wallerian变性的坐骨神经用植块法进行培养，通过相差显微活细胞计数和S－100细胞化学标记相结合鉴定了雪旺细胞增值和纯化程度。结果：通过上述方法获得大量雪旺细胞纯度达95％，并绘制其生长曲线，体外培养的雪旺细胞倍增时间为8d。结论：本方法能获得大量高纯度的雪旺细胞，为组织工程修复周围神经缺损打下基础。

Isolation, Purification and Cultivation of Schwann Cells from Adult Rabbit Sciatic Nerve in Vitro

Purpose To present an effective technique for culture and expansion of Schwann cells(SC)from adult rabit peripheral neres. Methods Adult predegenerated rabbit sciatic nerves culture in defined medium by primary cultures of adult SC.the purity of SC was identified through the phase contrast micros cope and S? 100 immunocytochemical staining. Results The best results were obtained with a conditioned medium supplemented with 20% fetal calf serum.In these conditions the purity of SC was about 95% in vitro .The growth curve was drawed.The doubling time of the 3rd passage of cells was 8 days. Conclusions These findings indicate that adult SC can be expanded from small preinjured nerve fragments in a short time period to provide a source of SC for tissue engineering cellular transplants.