应用亲和素——生物素——过氧化物酶复合物技术检测组织细胞中的禽呼肠孤病毒抗原

应用亲和素——生物素——过氧化物酶复合物(ABC)技术检测经不同途径接种ARV的鸡只,结果表明ARV抗原广泛分布于内脏实质器官、肠道、腱鞘和滑膜。在接种后4周除腱鞘和滑膜外,都检不到病毒抗原的存在,而这两种组织到试验结束时(60d)仍为阳性,感染鸡血液涂片的ABC检测结果均为阴性,ABC技术比病毒分离法更敏感。

USE OF THE AVIDIN-BIOTIN-PEROXIDASE COMPLEX (ABC) TECHNIQUE TO DETECT AVIAN REOVIRUS ANTIGEN

The avidin-biotin-peroxidase complex (ABC) technique was used to detect avian reovirus (ARV ) antigen in chickens inoculated with ARV via three routes. The test results suggested that the viral antigen wat widely distributed in the visceral parenchymal organs, intestinal tract, tendon sheaths and synovial membranes. Except for the tendon sheaths and synovial membranes, viral antigen was not detected 4 weeks after inoculation, but these two tissues still showed positive results when the 60-day experimental perkid terminated. No positive ABC staining was observed in the blood cell smears of infected chickens. The ABC method was more sensitive than vims isolation.