黄芩甙、地塞米松对大鼠感染性脑水肿细胞因子的影响

目的 :探讨黄芩甙、地塞米松对大鼠感染性脑水肿白细胞介素 1(IL 1β)、肿瘤坏死因子α(TNF α)的影响。方法 :应用大鼠感染性脑水肿模型 ,采用干湿法、原子吸收分光光度法、ELISA测定各组脑组织水含量、钠离子含量及脑匀浆上清液中IL 1β,TNF α水平的变化 ;在光镜下观察各组脑组织的细胞形态学改变。结果 :在大鼠感染性脑水肿(感脑 )组脑水含量、钠离子、IL 1β、TNF α含量均比生理盐水 (对照 )组明显增高 (P <0 .0 1)。在黄芩甙和地塞米松两组则上述指标均下降 ,低于感脑组 (P <0 .0 5 ) ;而两组间各指标比较差异无显著性 (P >0 .0 5 ) ;脑组织水含量与IL 1β ,TNF α含量成正相关 (r分别为 0 9381,0 8349,P <0 .0 1)。对照组脑组织结构正常 ,感脑组织脑组织可见弥漫的灶性水肿区 ;黄芩甙和地塞米松组水肿明显减轻。结论 :黄芩甙、地塞米松对大鼠感染性脑水肿有保护作用 ,效果相近 ;作用机制与抑制IL 1β ,TNF α过度产生有关

Effect of baicalin and dexamethasone on cytokines in brain tissue of infectious brain edema

Objective: To explore the effect of baicalin(BC) and dexamethasone(DXM) on interleukin 1β(IL 1β) and tumor necrosis factor α(TNF α) in brain tissues of infectious brain edema. Methods: The infectious brain edema model induced by pertussis bacilli(PB) was used. Brain water content(WC), content of Na + and the levels of IL 1β, TNF α were determined, and the morphological changes were observed under the light microscope. Results: The results showed that the levels of WC, Na +, IL 1β and TNF α in group PB were higher than those in group of normal saline(NS) (P<0.01). And they were lower in groups BC and DXM than those in group PB(P<0.05). In groups BC and DXM, there were no significant difference in the contents of water, Na +, IL 1β and TNF α(P>0.05). The water contents were positive linar correlated with IL 1β or TNF α(P>0.05). The water contents were positive linear correlated with IL 1β or TNF α contents(r=0.9381,0.8349,P<0.01). The structure of brain tissues in group NS was normal. In group PB, there were severe edema in brain tissues. In groups BC and DXM, the edema of brain tissues was reduced. Conclusions: The results suggest that the protective effect of BC and DXM against infectious brain edema were similar, the mechanism may be associated with the reduction of TNF α and IL 1β.