普伐他汀对激素性坏死股骨头内Cbfa1表达的影响

目的 研究普伐他汀对激素性坏死股骨头内Cbfal基因表达的影响．方法 54只新西兰白兔随机分为正常组（A组）、模型组（B组）和普伐他汀治疗组（C组），每组18只．B、C两组应用大肠杆菌内毒素及甲强龙造成股骨头坏死模型，C组于造模后4周开始用普伐他汀灌胃，A、B两组以等量蒸馏水灌胃．分别于喂药后第4、8、12周分批取兔股骨头行实时荧光定量PCR检测股骨头内Cbfal mRNA的表达．结果 B、C两组各时相点Cbfal mRNA的表达水平均低于A组，但C组喂药后第8、12周的表达量明显高于B组，差异有显著性（P〈0．01）．结论 普伐他汀可促进激素性坏死股骨头内Cbfal mRNA的表达，有可能成为临床治疗激素性股骨头坏死的有效药物．

The Effect of Pravastatin on Expression of Core Binding Factor Alphal 1 of Steroid Induced Necrosis of Femoral Head

Objective To investigate the effect of pravastatin on expression of core binding factor alphal 1 of steroid induced necrosis of femoral head.Methods 54 New Zealand white rabbits were randomly and equally divided into a normal control group(Group A),a model group(Group B) and a treatment group(Group C).All rabbits in Group B and Group C were injected lipopolysaccharide and methylprednisolone.Animals in Group C were treated with pravastatin at the 5th week,and the same volume of distilled water were given in Group A and Group B.Six rabbits in each group were killed at the 8th,12th and 16th week respectively.Femoral head specimens were studied by quantitative real-time PCR for detecting the expression of Cbfa1 mRNA.Results In Group B and Group C,Cbfa1 mRNA expression levels were lower than that of Group A,but the expression level of Group C increased significantly compared with Group B at the 12th and 16th week(P<0.01).Conclusion Pravastatin can improve the expression of Cbfa1,which is probably an effective clinical treatment of early steroid-induced necrosis of femoral head.