| 白花蛇舌草对H22肝癌细胞荷瘤组织bcl-2和bax蛋白表达的影响 |
| |
| 引用本文： | 胡玲,罗晓韵,唐纯志,谢宇晖,崔娜娟,陈苇菁,王洪琦. 白花蛇舌草对H22肝癌细胞荷瘤组织bcl-2和bax蛋白表达的影响[J]. 中国中西医结合消化杂志, 2011, 19(6): 378-381 |
| |
| 作者姓名： | 胡玲 罗晓韵 唐纯志 谢宇晖 崔娜娟 陈苇菁 王洪琦 |
| |
| 作者单位： | 1. 广州中医药大学,广东广州,510405
2. 广州中医药大学,广东广州510405;北京市中西医结合医院,北京100039 |
| |
| 基金项目： | 广东省"千百十工程"优秀人才基金 |
| |
| 摘 要： | [目的]观察H22肝癌细胞荷瘤组织bcl-2、bax蛋白的表达,探讨白花蛇舌草诱导热休克蛋白70（HSP70）表达及促进肝癌细胞凋亡的机制。[方法]将白花蛇舌草处理后的小鼠活体腹水H22肝癌细胞免疫小鼠,同时设党参对照,以HSP70单抗封闭后分为白花蛇舌草未封闭、封闭,党参未封闭、封闭及空白对照5组,然后以培养的H22肝癌细胞攻击小鼠形成荷瘤;免疫组化检测凋亡相关基因bcl-2、bax蛋白的表达。[结果]荷瘤组织bcl-2、bax表达定位于胞质。白花蛇舌草、党参未封闭和封闭组bcl-2、bax表达分别显著低于和高于空白对照组;白花蛇舌草、党参未封闭组bcl-2及bax表达也分别较封闭组下调和增高,但之间差异无统计学意义。与党参未封闭及封闭组比较,白花蛇舌草组bax表达增高,而bcl-2表达差异无统计学意义。[结论]白花蛇舌草促进H22肝癌细胞荷瘤组织细胞凋亡可能与上调bax蛋白表达相关。
|
| 关 键 词： | 白花蛇舌草 肝癌 bcl-2 bax 基因表达 |
Effect of herba hedyotis diffusae on bcl-2 and bax expression in bearing neoplasia of H22 hepatoma cells |
| |
| HU Ling,LUO Xiao-yun,TANG Chun-zhi,XIE Yu-hui,CUI Na-juan,CHEN Wei-jing,WANG Hong-qi. Effect of herba hedyotis diffusae on bcl-2 and bax expression in bearing neoplasia of H22 hepatoma cells[J]. Chinese Journal of Integrated Traditional and Western Medicine on Digestion, 2011, 19(6): 378-381 |
| |
| Authors: | HU Ling LUO Xiao-yun TANG Chun-zhi XIE Yu-hui CUI Na-juan CHEN Wei-jing WANG Hong-qi |
| |
| Affiliation: | HU Ling1,LUO Xiao-yun1,TANG Chun-zhi1,XIE Yu-hui1,CUI Na-juan1,2,CHEN Wei-jing1,WANG Hong-qi1(1Guangzhou University of TCM,Guangzhou 510405,China,2Integrated Traditional Chinese and Western Medical Hospital of Beijing City,Beijing 100039,China) |
| |
| Abstract: | [Objective]To observe the effect of herba hedyotis diffusae(HHD) on bcl-2,bax protein expression in neoplasia of H22 hepatoma cells,and study the mechanism of HHD in inducing HSP70 expression and promoting hepatoma cells apoptosis.[Methods]Mice were immunized with the H22 hepatoma cells from in-vivo ascites of the mice pretreated by HHD and Radix Codonopsis(RC).Mice were divided into five groups after being blocked with heat shock protein70(HSP70)monoclonal antibody:non-HHD blocking group,HHD blocking group... |
| |
| Keywords: | herba hedyotis diffuse(HHD) hepatoma bcl-2 bax |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
