| 基于DPPH、ABTS和FRAP的中药女贞子抗氧化谱效关系研究 |
| |
| 引用本文： | 李昌勤,姚辰,朱荣遥,黄勇鑫,康文艺,王金梅.基于DPPH、ABTS和FRAP的中药女贞子抗氧化谱效关系研究[J].中国中药杂志,2016,41(9):1670-1677. |
| |
| 作者姓名： | 李昌勤 姚辰 朱荣遥 黄勇鑫 康文艺 王金梅 |
| |
| 作者单位： | 河南大学 中药研究所, 河南 开封 475004;开封市保健食品功效成分研究重点实验室, 河南 开封 475004,河南大学 中药研究所, 河南 开封 475004,河南大学 中药研究所, 河南 开封 475004,河南大学 中药研究所, 河南 开封 475004,河南大学 中药研究所, 河南 开封 475004;开封市保健食品功效成分研究重点实验室, 河南 开封 475004,河南大学 中药研究所, 河南 开封 475004;开封市保健食品功效成分研究重点实验室, 河南 开封 475004 |
| |
| 基金项目： | 国家教育部大学生创新创业项目(201510475075);河南省高校科技创新团队项目(16IRTSTHN019);河南省教育厅科学技术研究重点项目(16A360008;15A360014);开封市创新人才项目(1509010);河南大学大学生创新计划项目(14NA017) |
| |
| 摘 要： | 运用HPLC测定不同加热条件处理的女贞子药材的特征图谱,通过DPPH、ABTS和FRAP法测定不同条件处理女贞子的抗氧化能力,运用偏最小二乘法进行数据分析,研究女贞子量化特征图谱和抗氧化活性的相关性,探讨女贞子抗氧化活性的物质基础,并筛选活性色谱峰。从35个匹配特征图谱中筛选出S_1,S_2,S15,S_(24),S_(27),S_(29),S_(32),S_(33),S_(35)与女贞子清除DPPH自由基能力呈显著正相关;S_1,S_2,S_5,S_8,S_(16),S_(27),S_(33)与女贞子清除ABTS自由基显著正相关;S_(12),S_(20),S_(22),S_(28),S_(30),S_(31),S_(32),S_(34)与女贞子还原Fe~(3+)能力显著正相关。其中S_1,S_2,S_(27),S_(33)与女贞子清除DPPH自由基、ABTS自由基均正相关,S_(32)与女贞子清除DPPH自由基、还原Fe~(3+)能力均正相关。通过与对照品比对确定了S_3为红景天苷、S_(19)为木犀草苷、S_(21)为特女贞苷、S_(22)为橄榄苦苷、S_(23)为女贞苷、S_(33)为木犀草素、S_(34)为芹菜素。试验结果表明特女贞苷和红景天苷等虽是规定的表征女贞子药材优劣的指标成分,但女贞子药效并不是靠个别成分含量决定。通过分析与药效相关的化合物群的含量才能真实反映药材的质量。
|
| 关 键 词： | 女贞子 抗氧化活性 偏最小二乘法 谱效关系 |
| 收稿时间： | 2016/1/31 0:00:00 |
Spectrum-effect relationship in antioxidant activity of Ligustri Lucidi Fructus based on DPPH, ABTS and FRAP assays |
| |
| LI Chang-qin,YAO Chen,ZHU Rong-yao,HUANG Yong-xin,KANG Wen-yi and WANG Jin-mei.Spectrum-effect relationship in antioxidant activity of Ligustri Lucidi Fructus based on DPPH, ABTS and FRAP assays[J].China Journal of Chinese Materia Medica,2016,41(9):1670-1677. |
| |
| Authors: | LI Chang-qin YAO Chen ZHU Rong-yao HUANG Yong-xin KANG Wen-yi and WANG Jin-mei |
| |
| Institution: | Institute of Chinese MateriaMedica, Henan University, Kaifeng 475004, China;Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China,Institute of Chinese MateriaMedica, Henan University, Kaifeng 475004, China,Institute of Chinese MateriaMedica, Henan University, Kaifeng 475004, China,Institute of Chinese MateriaMedica, Henan University, Kaifeng 475004, China,Institute of Chinese MateriaMedica, Henan University, Kaifeng 475004, China;Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China and Institute of Chinese MateriaMedica, Henan University, Kaifeng 475004, China;Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China |
| |
| Abstract: | Characteristic chromatogram of Ligustri Lucidi Fructus which were processed by heat treatment with different temperatures and times was developed by HPLC. DPPH, ABTS and FRAP assays were performed to determine the antioxidant activity. The spectrum-effect correlation was studied using Partial Least Squares(PLS)to explore the active ingredients of Ligustri Lucidi Fructus by investigating the relationship between HPLC spectrum and antioxidant activity. S1, S2, S15, S24, S27, S29, S32, S33 and S35were characteristic compounds in 35 matching characteristic chromatograms which were positive relationship with scavenging DPPH free radical activity. S1, S2, S5, S8, S16, S27 and S33were significantly positively related to scavenge ABTS free radical activity. S12, S20, S22, S28, S30, S31, S32 and S34were significantly positively related to restore Fe3+ activity. Among the chromatographic peaks, S1, S2, S27and S33were positively related to scavenge DPPH free radical and ABTS free radical activity, and S32was positively related to scavenge DPPH free radical and restore Fe3+ activity. S3, S19, S21, S22, S23, S33and S34 were determined as salidroside, luteolin-7-O-glucoside, specnuezhenide, oleuropein, ligustroflavone, luteolin andapigenin, respectively. The results showed that although specnuezhenide and salidroside were stipulated index compounds to evaluate the quality of Ligustri Lucidi Fructus, the Ligustri Lucidi Fructus pharmcodynamic effect did not depend on the contents of those index components. The quality of traditional Chinese medicines(TCMs)should be determined by the compound groups associated with pharmcodynamic effect. |
| |
| Keywords: | Lucidi Ligustri Fructus antioxidant activity partial least squares spectrum-efficacy |
| 本文献已被 CNKI 等数据库收录！ |
|
