p-MEK1/2与原发性高血压大鼠左心室肥厚关系的研究

目的动态观察p-MEK1/2在原发性高血压大鼠(SHR)左心室表达,进一步探讨MEK1/2-ERK1/2通路在高血压左心室重构中的作用。方法对比观察各周龄SHR大鼠与WKY大鼠血压,心脏/体重比值变化;免疫组织化学方法检测左心室p-MEK1/2的表达。结果整个实验过程中,WKY血压保持在正常水平,SHR16、SHR24组动脉收缩压均明显高于同周龄WKY组(P<0.05);与同周龄WKY相比SHR24组的心脏/体重比值增加明显(P<0.05);SHR24左心室相对壁厚度明显高于SHR8和WKY24大鼠(P<0.05);WKY和SHR大鼠左心室p-MEK1/2蛋白表达量均随着周龄的增加而增加,但SHR16、SHR24明显高于同周龄WKY(P<0.05)。结论 MEK1/2-ERK1/2通路可能有促进高血压左心室心肌肥厚发展的作用。

The Effect of ERK Signal Pathway on the Development of Left Ventricular Hypertrophy in Spontaneously Hypertensive Rats

Objective To observe dynamically expression of p-MEK1/2 in the left ventricle of spontaneously hypertensive rats(SHR) and to futher explore the role of MEK1/2-ERK1/2 pathway in hypertensive left ventricular remodeling.Methods The expression of p-MEK1/2 in the left ventricle were detected by immunohistochemistry.the blood pressure、heart/body weight in Wistar Kyoto(WKY)and SHR were observed.Results During the experiment blood pressure of WKY remained at normal level,and systolic blood pressure respectively in SHR16、SHR24 groups were significantly higher than those in the same week old WKY group(P<0.05);compared with the same week old WKY group,the heart/body weight ratio in SHR24 groups increased significantly(P<0.05);the left ventricular relative wall thickness of SHR24 was significantly higher than those of SHR8,SHR16and WKY24(P<0.05);the expression of p-MEK1/2 protein in left ventricle increased with the the week-old growth,and the expression of SHR16、SHR24 groups were higher than those of same week old WKY groups respectively(P<0.05).Conclusion MEK1/2-ERK1/2 pathway may promote the development of the left ventricular myocardial hypertrophy in hypertensive rats.