补益营卫方对衰老人皮肤成纤维细胞数量及细胞周期的影响

目的观察经补益营卫方药物血清作用后,衰老人皮肤成纤维细胞数量和细胞周期的变化。方法体外培养正常人皮肤成纤维细胞,将细胞分为年轻对照组、老龄对照组、补益营卫高剂量组、补益营卫中剂量组、补益营卫低剂量组。年轻对照组和老龄对照组用10%胎牛血清常规培养;补益营卫高、中、低剂量组分别用10%补益营卫方高、中、低剂量大鼠血清培养,五组细胞连续培养8d。通过细胞计数法观察细胞数量的变化;流式细胞术观察细胞周期。结果补益营卫高、中、低药物血清组的细胞数量和S期细胞比例均增高,且均高于年轻对照组和老龄对照组(P<0.05),以高剂量组增高比例明显。结论补益营卫方可促进衰老人皮肤成纤维细胞增殖(S期细胞比例增高),此作用有剂量依赖性。

Influence of the Recipe of Supplementing Ying - wei on the Number and Cell Cycle of the Aging Human Skin Fibroblasts

Objective To observe the influence of the drug serum of the recipe of supplementing Ying-wei on the number and cell cycle of the aging human skin fibroblasts Methods Normal human skin fibroblasts were cultured in vitro.Cells were divided into young control group,the aging control group,the high dose group of supplementing Ying-wei,middle dose group of supplementing Ying-wei,low dose group of supplementing Ying-wei.Conventional culture was used in the young control group and the aging control group with 10 % fetal bovine serum;High,medium and low dose groups of supplementing Ying-wei were cultured respectively with 10 % high,medium and low-dose groups,serum of the recipe of supplementing Ying-wei,the five groups of cells are cultured for eight days.cells’ number was observed through cell counting,the cycle of cell was observed by flow cytometry.Results Among supplementing Ying-wei drug serum with different levels of high,medium and low dose groups,the amount and the percentage of S phase cells in cell cycle of the aging human skin fibroblasts increased significantly,and they were significantly higher than those of young control group and the aging control group(P < 0.05).Conclusion The recipe of supplementing Ying-wei can promote the proliferation of the aging skin fibroblast(the ratio of S phase cell is increased),and this function has dose dependent.