应用cDNA-AFLP研究甘草不同变异类型特异表达的基因

目的:探讨生态环境和遗传背景相同条件下8种不同变异类型甘草在基因表达水平上的差异,旨在揭示甘草种内变异的分子机制.方法:采用cDNA-AFLP、生物信息学等方法,对不同变异类型甘草在生长旺盛期根部组织特异表达的基因进行分析和功能预测;采用反相Northern杂交对特异表达的基因进行验证.结果:共获得14条差异表达基因片段,有6个基因序列编码的蛋白功能未知,其余的基因片段编码的蛋白参与了植物的抗菌,调节生物体内代谢,增加植物的抗热(高温)性,提高植物体固氮能力等生物过程.结论:首次克隆了不同变异类型甘草特异表达的基因,为筛选优良变异类型、品种选育、功能基因组的研究奠定基础.

Identification of differentially expressed genes in different anomalies of Glycyrrhiza uralensis by cDNA AFLP method

Objective: To understand the molecular mechanism in genetic variation within species, and identify the differentially expressed genes among 8 anomalies of Glycyrrhiza uralensis under the same genetic background and cultivated environment. Method: The differentially expressed genes in roots of different anomalies of G. uralensis in vigorous growing stage were identified by cDNA-AFLP and confirmed by reverse Northern hybridization. Their functions were inferred through bioinformatics method. Result: Fourteen differentially expressed genes were identified, among which, the function of 6 were unknown, and the rest were involved in antibacterium, regulating metabolism, enhancing the heat resistance ability, increasing the nitrogen fixation ability, and other bioprocesses. Conclusion: For the first time, the differentially expressed genes were cloned from the different anomalies of G. uralensis. This provided the basis for the screening of fine anomalies and varieties, and the research of functional genomics.