大鼠局灶性脑缺血再灌注后脑和肝、肾Bax、Bcl-2的蛋白表达

目的研究大鼠急性局灶性脑缺血再灌注后脑和肝、肾细胞凋亡和Bax、Bcl-2的蛋白表达变化,探讨急性局灶性脑缺血再灌注后对肝和肾脏的影响。方法参照Longa等的线栓法采用阻断大鼠大脑中动脉后再灌注（MCAO）,按随机原则将54只Wistar大鼠分为3个组：正常对照组（n=6）、假手术组（n=6）及缺血再灌注后2h、6h、12h、24h、48h、72h、5d 7个亚组,应用免疫组织化学SP染色技术检测脑和肝、肾Bax、Bcl-2的蛋白表达,并计数计算出平均阳性率（LI）,同时检测了肝和肾功能改变。结果（1）大鼠脑缺血再灌注后各时相点的肝、肾脏组织均有不同程度的病理损害；（2）Bax、Bcl-2在手术组的大鼠中呈明显的阳性表达,与正常对照组和假手术组之间比较有非常显著性差异（P＜0．01,P＜0．001）。结论大鼠急性局灶性脑缺血再灌注后肝、肾功能减退并出现组织细胞凋亡明显增加。

A study of expression of Bax and Bcl-2 in liver and kidney after acute focal ischemia-reperfusionin rat

Objective To investigate expression of Bax and Bcl-2 gene in brain,lung and intestines after a- cute focal ischemia-reperfusion in rat.To discuss the effect on liver and kidney after acute focal ischemia-reperfu- sion in rats.Methods The acute focal ischemia-reperfusion model in rats was made by occluding middle cerebral artery(MCA)with thread following Longa for two hours and followed by reperfusion.Fifty-four Wistar rats were randomly divided into three groups:normal control group(n=6),sham-operative group(n=6),operative group(n =42)including2h,6h,12h,24h,48h,72h,5d seven time points.Expression of Bax and Bcl-2 in brain,liver and kid- ney were detected by streptavidin peroxidase.The positive cells were counted under light miscroseope and then the labeling index(LI)were calculated and the function of liver and kidney were assayed.Results(1)Brain,liver,kid- ney at each time point after ischemia in rats were observed injuries in varying degrees.(2)Bax and Bcl-2 positive cells in the operative group were significantly increased.There was significant difference between the operative group and non-operative group(P<0.01,P<0.001).Conclusion The function of liver and kidney decrease and apoptosis of brain,liver and kidney increase after acute focal ischemia-reperfusion.