妊娠相关糖蛋白对E型选择素介导的内皮细胞黏附作用研究

目的：本实验旨在探讨高度纯化的羊水和孕 妇血清人绒毛膜促性腺激素(human chrionic gonadotropin,hCG)和glycodelin对E型选择 素介导的HepG2细胞与内皮细胞黏附过程的影响。方法:以色谱仪分离和 纯化羊水和血清的hCG和glycodelin，进一步测定［51Cr］标记的肝癌细胞系-HepG 2细胞在不同来源、不同浓度hCG和glycodelin作用下和经过白细胞介素-1β（IL-1β）刺激 后表达E型选择素的内皮细胞的黏附能力，从而评价这两种糖蛋白对E型选择素介导的内皮 细胞黏附过程的影响。结果：羊水和血清hCGs能有效抑制E型选择素介导 的内皮细胞黏附，其抑制作用呈浓度依赖关系,IC50分别为1.5×10-7 mol/L 和2.1×10-7 mol/L，相对抑制力分别为纯四糖结构sialyl-Lewisx（sLex）的1 .5×104和1.1×104倍；羊水和血清glycodelin同样有此作用，其IC₅₀分别为3 .2×10-6 mol/L和6.3×10-6 mol/L，相对抑制力分别为sLex的7.2×10 2和3.7×102倍；纯四糖结构sialyl-Lewisx（sLex）的IC₅₀为2.3×10 -3 mol/L。结论:妊娠相关糖蛋白hCG和glycodelin是E型选择素 介导的内皮细胞黏附的有效抑制物。

Effect of pregnancy-associated glycoproteins on E-selectin-medi ated endothelial cell adhesion

AIM:To explore the effect of high purified human chorionic gonadotropin (hCGs) and glycodelins isolated from amniotic flui d and serum of pregnant women on E-selectin mediated endothelial cell adhesion.METHODS:The hCGs and glycodelins in human amniotic fluid and se rum collected from pregnant women were purified by using chromatography.The bin ding of51Cr］-labelled HepG2 cells to IL-1β stimutated human umbilical vein endothelial cells (HUVECs) in the presence of increasing concentrations of hCGs and glycodelins was quantified.The effect of hCG and glycodelin on E-selec tin-mediated endothelial cell adhesion was observed.RESULTS:HCGs isolated from amniotic fluid and serum of pregnant women inhibited the E-selectin mediated adhesion of HepG2 cells to HUVECs in a dose-dependent manner,the IC₅₀ was 3.2×10-6 mol/L and 6.3×10 -6 mol/L,respectively and the relative inhibition was 1.5×104 and 1 .1×104,respectively,compared to sialyl-Lewisx.The IC₅₀ of amnioti c fluid glycodelin and serum glycodelin was 3.2×10-6 mol/L and 6.3×10 -6 mol/L respectively,and the relative inhibition was 7.2×102 and 3.7×102,respectively,compared with sialyl-Lewisx.CONCLUSION:Pregnancy-associated glycoproteins,hCG and glycodel in,are the powerful inhibitors for E-selectin-mediated endothelial cell adhesion.