吗啡延迟预处理对大鼠心肌缺血再灌注损伤细胞色素C氧化酶的影响

[目的]探讨吗啡延迟预处理对大鼠心肌缺血再灌注损伤的保护机制.[方法]40只大鼠随机分成4组：假手术组（C组）、缺血再灌注组（I/R组）、吗啡预处理组（M组）,吗啡预处理＋阿片类受体阻断剂组（N组）,每组10只.C组仅行左冠脉套线而不阻断150 min;I/R组行左冠脉阻断30 min,再灌注120 min;M组静注吗啡0.3mg/kg,24 h后处理同I/R组;N组在静注吗啡前10 min,静注阿片类受体阻断剂纳洛酮6mg/kg,其余处理同I/R组.再灌注120min后,免疫印记法测心肌细胞色素C氧化酶（cytochrome c oxidase,CcO）表达水平,同时测心梗面积.[结果]与C组相比,I/R组、M组和N组CcO表达水平均降低;与I/R组比,M组CcO表达水平增高,心肌梗死面积减少,且差异有显著性（P＜0.05）.[结论]吗啡延迟预处理对心肌缺血再灌注损伤的保护作用可能与促进心肌CcO表达有关.

Effect of Morphine Delayed Preconditioning on Cytochrome C Oxidase in Rat Myocardial Ischemia Reperfusion Injury

[Objective]To explore the protective mechanism of morphine delayed preconditioning on myocardial ischemia reperfusion injury in rats.[Methods] A total of 40 rats were randomly divided into sham operation group（group C）,ischemia reperfusion group（group IR）,morphine preconditioning group（group M） and morphine preconditioning＋opioid receptor inhibitor group（group N） with 10 rats in each group.Group C only received the overlapping line of left coronary artery without occlusion for 150min.Group IR received occlusion of left coronary artery for 30min and then reperfusion for 120min.Group M was given intravenous injection with morphine 0.3mg/kg and the same treatment as IR group after 24h.Group N was given intravenous injection with opioid receptor inhibitor naloxone 6mg/kg at 10min before intravenous injection of morphine and then the same treatment as group IR.After 120min reperfusion,the expression of cytochrome C oxidase（CcO） in myocardium was detected by Western blotting.At the same time,myocardial infraction area was measured.[Results] Compared with group C,the expressions of CcO in group IR,M and N were decreased.Compared with group IR,the expression of CcO was increased and myocardial infarction area was decreased in group M,and there was significant difference（P ＜0.05）.[Conclusion] Morphine delayed preconditioning has the protective effect on myocardial ischemia reperfusion injury,which may be related with the increasing of the expression of CcO in myocardium.