番禺地区无偿献血者酶免阴性样本的核酸检测结果分析 |
| |
引用本文: | 谢敬文,蓝文莉,黎淑贞,陈筱颖.番禺地区无偿献血者酶免阴性样本的核酸检测结果分析[J].临床输血与检验,2014,16(4):355-357. |
| |
作者姓名: | 谢敬文 蓝文莉 黎淑贞 陈筱颖 |
| |
作者单位: | 511400,广东省广州市番禺区中心血站 |
| |
摘 要: | 目的通过对酶联免疫吸附实验(ELISA)反应阴性样本的核酸检测技术(NAT)的筛查结果进行分析,探讨核酸检测技术在血液筛查中的应用价值。方法先采用ELISA试剂分别对献血者标本进行HBsAg、抗-HCV和抗-HIV筛查,筛查结果阴性的样本,再用罗氏诊断cobas s 201系统进行HIV、HCV和HBV三项联合NAT检测,并对NAT联检阳性的标本进行分项确证实验。结果在8 147例经ELISA筛查阴性血液标本中检出6例NAT阳性标本,经分项确证6例均为HBV DNA阳性,阳性检出率约为0.074%。结论 ELISA筛查阴性样本中仍存在一定的HBV DNA阳性结果,采用ELISA联合NAT筛查策略,能有效降低输血感染乙肝病毒的风险。
|
关 键 词: | 血液筛查 酶联免疫吸附实验 核酸检测 |
Analysis on Nucleic Acid Test Results of ELISA-negative Blood Donors from Panyu District |
| |
Institution: | XIE Jing-wen, LAN Wen-li ,LI Shu-zhen ,et al. (Panyu Blood Centre of Guangzhou, Guangzhou 511400) |
| |
Abstract: | Objective By analyzing the nucleic acid test (NAT) results of ELISA-negative blood samples, probe the application value of nucleic acid detection technique in blood screening. Methods First use of enzyme-linked immunosorbent assay (ELISA) reagent specimens of blood donors were HBsAg, anti-HCV and anti-HIV screening, negative samples ,with Roche Diagnostics Cobas s 201 system ,HIV,HCV and HBV were three joint detected, and the joint detected positive samples were breakdown confirmed. Results In the 8147 ELISA-negative samples, 6 samples were NAT positive, the breakdown confirmatory test show that all the 6 eases were HBV-DNA positive, the positive rate is about 0. 074 %. Conclusion ELISA-negative samples still exist in some HBV-DNA positive risk, Use ELISA joint NAT in blood screening can effectively reduce the risk of transfusion infected with hepatitis B virus. |
| |
Keywords: | Blood screening Enzyme-linked immunosorbent assay Nucleic acid test |
本文献已被 维普 万方数据 等数据库收录! |
|