甘草甜素对HSV-1抑制作用的实验研究

目的:观察甘草甜素对单纯疱疹病毒Ⅰ型的抑制作用,为临床更为有效的治疗单纯疱疹病毒性脑炎探索新的药物.方法:体外培养Vero细胞,分成甘草甜素在病毒吸附vero细胞后加入病毒、吸附前甘草甜素预处理细胞以及吸附前甘草甜素预处理病毒,通过观察空斑形成单位来评价甘草甜素对单纯疱疹病毒Ⅰ型的抑制作用,以及作用发生于病毒感染的哪个阶段.结果:病毒吸附vero细胞后加入甘草甜素能明显抑制病毒所致的空斑形成,IC50为0.56mmol/L.吸附前甘草甜素预处理细胞以及吸附前甘草甜素预处理病毒,对病毒所致的空斑形成无明显影响.结论:甘草甜素能明显地抑制单纯疱疹病毒Ⅰ型的复制,它的作用是发生在病毒穿入细胞后的阶段,不能阻止病毒对细胞的吸附,也不能直接灭活病毒.

Experimental study on inhibitory effect of glycyrrhizin on HSV-1 in vitro

Objective:To investigate the inhibitory effect of glycyrrhizin on HSV-1 in vitro,and to search a new drug against herpes simplex encephalitis.Methods:The Vero cells were cultured and divided into three groups:the group treated with glycyrrhizin after absorption of HSV-1 into the Vero cells,the group of pretreated cells and the group of pretreated HSV-1 with both glycyrrhizin before absorption of HSV-1 into the Vero cells.The plaque formation units were observed to evaluate the inhibitory effect of glycyrrhizin on HSV-1 and the stage when the virus replicating was inhibited.Results:Glycyrrhizin treatment after the absorption of virus into the cells obviously reduced the plaque formation units due to the virus infection and IC 50 was 0.56mM.Pretreated cells and pretreated HSV-1 with glycyrrhizin before absorption of HSV-1 into the cells did not reduce the plaque formation units.Conclusion:Glycyrrhizin can markedly inhibit the replication of HSV-1 in Vero cells.Glycyrrhizin can not inhibit the virus absorption and inactivate the virus.