| CRKL基因对K 562细胞的作用研究 |
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| 引用本文： | 王莎,孙秉中,于文强. CRKL基因对K 562细胞的作用研究[J]. 临床血液学杂志, 2002, 15(4): 168-170 |
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| 作者姓名： | 王莎 孙秉中 于文强 |
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| 作者单位： | 第四军医大学西京医院血液科 西安710032(王莎，孙秉中)，第四军医大学西京医院血液科 西安710032(于文强) |
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| 基金项目： | 国家自然科学基金资助项目 (NO .39770 336 ) |
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| 摘 要： | 目的：通过研究CRKL基因反义寡核苷酸（AODN）对K562细胞增殖及凋亡的作用。探讨CRKL基因对慢性粒细胞性白血病（CML）发病的影响。方法：应用CRKL基因反义寡核苷酸作用于K562细胞，通过^3H-TdR掺入，流式细胞术，Rt-PCR等方法观察K562细胞形态学，细胞周期，细胞动力学及基因表达等变化。结果：CRKL基因ASODN对K562细胞的增殖有抑制作用。并可诱导K562细胞的凋亡。结论：CRKL基因在CML的发病中可能有重要作用。
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| 关 键 词： | 反义寡核苷酸 CRKL基因 K562细胞 慢性粒细胞性白血病 |
| 文章编号： | 1004-2806(2002)04-0168-03 |
| 修稿时间： | 2001-12-10 |
The role of CRKL gene in K 562 cell lines |
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| WANG Sha SUN Bingzhong YU Wenqiang. The role of CRKL gene in K 562 cell lines[J]. Journal of Clinical Hematology, 2002, 15(4): 168-170 |
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| Authors: | WANG Sha SUN Bingzhong YU Wenqiang |
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| Affiliation: | WANG Sha 1 SUN Bingzhong 1 YU Wenqiang 1 |
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| Abstract: | Objective:To investigate the effects of CRKL gene antisense-oligonucleotides(ASODN) on cell proliferation and apoptosis in K 562 cell line and thus assess the effect of CRKL gene in the pathogenesis of CML.Method: K 562 cells were cultivated with CRKL oligos which were mediated by cationin liposome. The cell viability, proliferation, apoptosis, cell cycle and gene expression were examined by 3 H-TdR doping , FCM and RT-PCR.Result: CRKL-ASDON could inhibit the proliferation of K 562 cells and induce apoptosis of K 562 cells.Conclusion: CRKL gene may play a key role in the pathogenesis in Ph-positive CML. |
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| Keywords: | Antisense-oligonucleutide CRKL gene K 562 Cell Chronic mylocytic leukemia |
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