高糖状态对大鼠肺泡巨噬细胞释放NO和TNF的影响

从正常Ｗｉｓｔａｒ大鼠肺中分离出肺泡巨噬细胞，在体外正常和高糖状态下培养并分别以干扰素（ＩＦＮａ－２ｂ、卡介苗（ＢＣＧ）以及干扰素和卡介苗联合刺激肺泡巨噬细胞，测定其释放一氧化氮（ＮＯ）和肿瘤坏死因子－ａ（ＴＮＦ－ａ）量。结果显示，高糖状态下ＢＣＧ刺激肺泡巨噬细胞时。其ＮＯ和ＴＮＦ－ａ释放量明显低于对照组（Ｐ＜０．０１）：ＩＦＮａ－２ｂ刺激肺泡巨噬细胞时，其ＮＯ和ＴＮＦ－ａ释放量与对照组无差别（Ｐ＞０．０５）：ＩＦＮａ－２ｂ与ＢＣＧ协同刺激肺泡巨噬细胞时，其ＮＯ和ＴＮＦ－ａ释放量明显低于对照组（Ｐ＜０．０１）。由此表明高糖状态下。肺泡巨噬细胞释放ＮＯ和ＴＮＦ－ａ减少，可能是导致糖尿病患者肺部易感染的一个重要原因。

Effect of high glucose level on release of nitric oxide and tumor necrosisfactor-a in rats alveolar macrophages

The alveolar macrophages were isolated from Wistar rat lung, and cultured in normal and high glucose condition, and then stimulated respectively, with IFNx - 2b, BCG and the combination of IFNa - 2b and BCG. The concentration of nitric oxide(NO) and tumor necrosis factor - a (TNF - a) in cultured rat alveolar macrophages was evaluated. The results showed that the volume of NO and TNF - a produced by alveolar zaacrophages after stimulation with BCG was aignificantly lower in the high glucose group than in the control ( P< 0.01), and the volume of NO and TNF- a produced by alveolar macrophagea after stimulation with IFNa - 2b was not different between the high glucose group and the control group (P>0.05). Under the effect of IFNa - 2b and BCG, the volume of NO and TNF - a produced by alveolar macrophages in high glucose conditions decreased significantly in comparison with control group. These data indicate that the decrease of NO and TNF - a produced by alveolar macrophages in high glucose condition might be an important factor enhancing the development of pulmonary infection of diabetes mellitus.