Choline acetyltransferase and acetylcholinesterase activities in muscles of aged mice |
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| Authors: | H Washio C Imazato-Tanaka K Kanda S Nomoto |
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| Affiliation: | 1. Cardiovascular Research Institute, Baylor College of Medicine, Houston, TX 77030, USA;2. Dept. of Molecular Physiology & Biophysics, Baylor College of Medicine, Houston, TX 77030, USA;3. Dept. of Medicine/Cardiology, Baylor College of Medicine, Houston, TX 77030, USA;1. Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, Brazil;2. Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo, Brazil;3. Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, USA;4. Department of Science and Technology, Federal University of São Paulo (ICT-UNIFESP), São José dos Campos, SP, Brazil |
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| Abstract: | The activities of choline acetyltransferase (CAT) and acetylcholinesterase (AChE) were assayed in intact diaphragm, extensor digitorum longus (EDL), and soleus muscles or their homogenates of young (2-6 months) and aged (24-34 months) mice. CAT activity (per mg of protein) was significantly higher in diaphragm and soleus of old mice in comparison with the young but the age change in EDL was negligible. On the other hand, AChE activity (per mg of protein) was significantly higher in EDL of old mice but in diaphragm and soleus muscles the enzyme activity did not show any significant change statistically. The diaphragm muscle was divided into two fractions, one being neuromuscular (NM) fraction and the other the remainder of the muscle (M fraction). No appreciable change in the ratio of the enzyme activities of NM fraction to the one of M fraction was obtained between the young and aged preparations. Thus, it seems likely that there is an age-related change in CAT and AChE activities which might be affected by the degree to which muscle activity is maintained. |
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