2-甲氧基雌二醇对复发难治性骨髓瘤患者骨髓瘤细胞作用的实验研究

目的探讨2-甲氧基雌二醇（2-ME）对复发性难治性骨髓瘤患者骨髓瘤细胞增殖和凋亡的影响。方法对复发性难治性骨髓瘤细胞进行分离、培养,用不同剂量的2-ME处理不同的时间,然后用四甲基偶氮唑蓝法检测细胞活力和流式细胞分析细胞周期分布和细胞凋亡率。结果随着2-ME剂量的增加和作用时间的延长,细胞抑制率和凋亡率增加;G0/G1期细胞比例增加,而S期细胞减少,各组之间差异有显著性（〈0.05）。结论2-ME对复发难治性骨髓瘤患者的骨髓瘤细胞具有抑制细胞增殖、促进细胞凋亡的作用。

Experimental study of 2-methoxyestradiol on proliferation and apoptosis of relapse and refractory multiple myeloma cells

Objective To investigate the effects of 2-methoxyestradiol on the growth and apoptosis of multiple myeloma cells in vitro.Methods The relapsed and refractory multiple myeloma patient＇s cells were separated and cultivated,which were treated by different doses of 2-methoxyestradiol for different time,while the cells in the validity was deter-mined by MTT assay,and the cell-cycle and apoptosis were determined by flow cytometry.Results With 2-methox-yestradiol doses increased and time prolonged,the cells were arrested at G0/G1 stages which S stages decreased,accom-panied with the enhanced apoptosis peak and percentage.There was a difference between the control group and 2-meth-oxyestradiol treatment groups（〈0.05）.Conclusion 2-methoxyestradiol can inhibit proliferation and induce apoptosis of the relapsed and refractory multiple myeloma patient＇s cells.