| 人白细胞介素17cDNA的克隆及表达 |
| |
| 引用本文： | 郭莉 刘钟滨. 人白细胞介素17cDNA的克隆及表达[J]. 同济大学学报(医学版), 2002, 23(5): 370-372 |
| |
| 作者姓名： | 郭莉 刘钟滨 |
| |
| 作者单位： | 同济大学医学院微生物学与免疫学教研室,上海,200331 |
| |
| 基金项目： | 铁道部科技基金资助项目 (B0 0 40 ) |
| |
| 摘 要： | 目的 获取重组的人白细胞介素17以进行科学研究和应用。方法 应用RT－PCR的方法，从经PHA活化的人外周血单个核细胞中扩增hIL-17cDNA的编码序列，将其克隆至测序载体pBS SKⅡ（ ）中进行测序，再将其亚克隆至表达载体pBV220中，在大肠杆菌XL1－Blue中进行表达。结果 克隆获得了hIL-17cDNA，经热诱导后使hIL-17蛋白在大肠杆菌中的表达量达到了39．7％。结论 通过克隆hIL-17cDNA的编码序列及热诱导使hIL-17蛋白在大肠杆菌中得到了高效表达。
|
| 关 键 词： | 人白细胞介素7 基因克隆 基因表达 RT－PCR法 细胞因子 |
| 文章编号： | 1008-0392(2002)05-0370-03 |
| 修稿时间： | 2002-01-18 |
The Cloning and Expression of cDNA Encoding Human IL-17 |
| |
| GUO Li,LIU Zhong bin. The Cloning and Expression of cDNA Encoding Human IL-17[J]. Journal of Tongji University(Medical Science), 2002, 23(5): 370-372 |
| |
| Authors: | GUO Li LIU Zhong bin |
| |
| Abstract: | Objective To obtain recombinant human interleukin 17(hIL?17) for science research and application.Methods The complete encoding region of hIL?17 gene was cloned from activated human peripheral blood mononuclear cells by RT?PCR and a recombinant plasmid pBS?hIL?17 was constructed for sequencing.Then hIL?17 cDNA was subcloned into vector pBV220 to express hIL?17 protein in E.Coli XL1?Blue.Results The hIL?17cDNA was cloned and the hIL?17 protein was highly expressed in E.Coli up to 39.7% of total bacterial protein by temperature inducing.Conclusion The hIL?17cDNA was cloned successfully and hIL?17 protein can be highly expressed in E.Coli by temperature inducing. |
| |
| Keywords: | human interleukin?17 cDNA gene cloning expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《同济大学学报(医学版)》浏览原始摘要信息 |
|
点击此处可从《同济大学学报(医学版)》下载全文 |
