Mechanisms of vancomycin-induced histamine release from rat peritoneal mast cells |
| |
Authors: | Yasufumi Horinouchi Kihachiro Abe Keiji Kubo Masuichiro Oka |
| |
Institution: | (1) Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812 Fukuoka, Japan;(2) Department of Dental Anesthesiology, Faculty of Dentistry, Kyushu University, 3-1-1 Maidashi, Higashi-ku, 812 Fukuoka, Japan |
| |
Abstract: | The mechanisms of vancomycin (VCM)-induced histamine release were studied with rat peritoneal mast cells. VCM (>1×10–3
M) released histamine from the isolated mast cells in a dose-dependent and noncytotoxic manner. In the absence of extracellular Ca2+, the histamine release was reduced markedly. When the intracellular Ca2+ was depleted, it was further decreased. The Fura-2-loaded single mast cells showed a biphasic increase in the intracellular Ca2+ concentration (Ca2+]i) by VCM: the first transient and the second sustained components. In the absence of extracellular Ca2+, the transient component was unchanged, while the sustained component was eliminated completely. The IP3 content in the mast cells increased within 10 s after the application of VCM. These results suggest that VCM releases histamine from rat peritoneal mast cells via an IP3 production and increase in Ca2+]i. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|