| 比较牛颈静脉去细胞处理实验方法 |
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| 引用本文： | 陆军,肖学钧,陈欣欣.比较牛颈静脉去细胞处理实验方法[J].岭南心血管病杂志,2008,14(6). |
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| 作者姓名： | 陆军 肖学钧 陈欣欣 |
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| 作者单位： | 1. 东莞市人民医院心胸外科,广东,东莞,523018
2. 广东省人民医院心外科,广州,510100 |
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| 摘 要： | 目的比较两种牛颈静脉脱细胞方法。方法改良组对牛颈静脉使用0.25%聚乙二醇辛基苯基醚、2.5g/L脱氧胆酸钠、0.2g/L乙二胺四乙酸、0.1g/LRNA酶和0.2g/LDNA酶作48h脱细胞处理;常规组使用0.5g/L胰蛋白酶和0.2g/L乙二胺四乙酸处理24h,再按以上处理组程序处理24h。血管壁和瓣膜自身细胞脱除和细胞外基质改变在染色后用扫描电镜观察;血管组织强度用生物力学检测。结果改良处理组管壁和瓣膜自身细胞完全脱除而细胞外基质无明显改变,常规组仍有大量固有细胞残留,弹力纤维出现断裂;两组脱细胞血管的生物力学性能无明显变化。结论改良脱细胞处理用聚乙二醇辛基苯基醚、脱氧胆酸钠、乙二胺四乙酸、RNA酶和DNA酶组合,是一种较理想的牛颈静脉脱细胞方法。
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| 关 键 词： | 牛颈静脉 脱细胞支架 组织工程 |
Comparison of decellularization procedures for bovine jugular veins |
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| LU Jun,XIAO Xue-jun,CHEN Xin-xin.Comparison of decellularization procedures for bovine jugular veins[J].South China Journal of Cardiovascular Diseases,2008,14(6). |
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| Authors: | LU Jun XIAO Xue-jun CHEN Xin-xin |
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| Institution: | LU Jun1,XIAO Xue-jun2,CHEN Xin-xin2(1Department of Cardio-thoracic Surgery,Dongguan People\' s Hospital,Dongguan Guangdong 523018,China,2Department of Cardiac Surgery,Guangdong Provincial Gernal Hospital,Guangzhou 510100,China) |
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| Abstract: | Objectives To compare decellularization procedures for bovine jugular veins.Methods Fresh bovine jugular veins were decellularized.In improved group,veins were treated with 0.25% Triton X-100,sodium-deoxycholate,0.2 g /L EDTA,0.1 g /L RNase and 0.2 g /L DNase for 48 h,while in regular group,veins were treated with 0.5 g /L trypsinase and 0.2 g /L EDTA for 24 h,followed by 0.25% Triton X-100,sodium-deoxycholate,0.2 g /L EDTA,0.1 g /L RNase and 0.2 g /L DNase for 24 h.Residual cells and extracellular matrix c... |
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| Keywords: | Bovine jugular veins Decellularized scaffold Tissue engineering |
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