Study on Oxygen Supply and Protection of Bone Marrow in Acute Radiation injured Mice

Itwasreportedthatacuteradiationcoulddestroythemurinebonemarrowmicrovesselsystemandledtostasisofbloodll].WeareleadtoasksuchquestionaswhatchangeswouldtakeplaceastotheOxygensupplyofbonemarrowmicroenviromentinthissituation.InviewoftheChinesetraditionaltheoryofblood--activatingandstasis--eliminating,weobservedtheeffectsofcommonlyusedblood--activatingandstasis-eliminatingdrug(acompoundpreparationcomposedofredsageroot,Chineseangelicaroot,Chuanxiongrhizomeandredpeomyroot)ontherehabilitationandoxygens…

Study on oxygen supply and protection of bone marrow in acute radiation injured mice

Summary After irradiated by 8 Gy⁶⁰Co γ-ray, mice were intraperitoneally injected immediately with 0. 2 ml 100 % compound blood-activating soup twice a day for 10 days. The in situ ulnar bone marrow partial pressure of oxygen (PbO₂) was determinedin vivo before, during and after irradiation respectively. The bone marrow sections in the same part were observed. Our results showed that the normal murine ulnar P_bO₂ was 12. 72±1. 05 kPa. During irradiation, the level of P_bO₂ decreased to 10. 78±1. 17 kPa (P<0. 001). And 3 days after irradiation, P_bO₂ decreased to 9. 75±0. 52 kPa, suggesting that the commonly used “blood-activating and stasis-eliminating” Chinese drugs could promote the rehabilitation and proliferation of bone marrow microvessels in the acute radiation injured mice, expand their areas, increase the oxygen supply of bone marrow microenviroment, thereby leading to P_bO₂ much higher increase than that of control group. It is also helpful in the proliferation and rehabilitation of hematopoietic cells. This project was supported by the National Natural Science Foundation of China (Serial No. 39270637) and Provincial Health Department.