Fe2O3纳米磁流体热疗治疗肝癌

目的　研究在一定高频交变磁场不同浓度的Fe2 O3 纳米磁流体热疗对SMMC772 1肝癌的治疗作用。方法　光学显微镜、电子显微镜观察细胞形态 ,四甲基偶氮唑蓝 (MTT )比色法检测Fe2 O3 纳米磁流体热疗 (MFH)对SMMC772 1肝癌细胞株生长的影响 ,流式细胞仪 (FCM )检测凋亡细胞 ,荷瘤鼠热疗实验检测MFH对肝癌的肿瘤体积抑制率及质量抑制率。结果　SMMC772 1细胞经Fe2 O3 纳米磁流体热疗作用后 ,细胞增殖受到明显抑制 ,细胞凋亡率明显增加 ,且与磁流体浓度呈依赖关系 ;动物实验显示纳米磁流体热疗对肝癌的体积和质量有明显的抑制作用。结论　Fe2 O3 纳米磁流体热疗能抑制肝癌SMMC772 1细胞增殖 ,促进其凋亡 ,并对肝癌显示明显的体积和质量抑制效应 ,对肝癌的治疗有一定的作用。

Study on the therapeutic effect of Fe2O3 nanometer magnetic fluid hyperthermia on liver cancer

Objective To study the therapeutic effect of Fe 2O 3 nanometer magnetic fluid hyperthermia under a high frequency alternating electromagnetic field on human hepatocarcinoma SMMC 7721 cells in vitro and in vivo.Methods By light and electron microscopy,the morphologic changes of cells were observed.By means of MTT reduction assay,the effect of Fe 2O 3 nanometer magnetic fluid hyperthermia on the proliferation of SMMC 7721 cells was measured.Ratio of apoptotic cell was detected by flow cytometry and the ratio of weight and volume inhibition to liver cancer with magnetic fluid hyperthermia was detected by hyperthermatic experiment on nude mice with tumor.Results Fe 2O 3 nanometer magnetic fluid hyperthermia could significantly inhibit the proliferation and rapidly increase the ratio of apoptosis of SMMC 7721 cells and the effect was dose dependent,and could also induce a G 2/M phase arrest in this cell line.Animal experiment showed that it could significantly inhibit the weight and volume of liver cancer.Conclusion Fe 2O 3 nanometer magnetic fluid hyperthermia could inhibit the proliferation and promote apoptosis of human hepatocarcinoma SMMC 7721cells in a concentration dependent manner.Animal experiment revealed that Fe 2O 3 nanometer magnetic fluid hyperthermia could significantly inhibit weight and volume of liver cancer.It could take a part role in the treatment of liver cancer.