白细胞介素10基因多态性与胃癌恶病质的关系

目的 探讨白细胞介素10(IL-10)基因启动子区-1082、-819和-592位点的多态性及单体型与胃癌恶病质的关系.方法 将223例胃癌患者分为恶病质组107例,非恶病质组116例.用放射免疫学方法检测两组患者的血清IL-10水平,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测两组患者IL-10基因-1082、-819和-592位点的单核苷酸多态性.结果 恶病质组血清IL-10水平为(91.64±13.25)ng/ml,较非恶病质组(56.53±12.43)ng/ml]显著升高(P＜0.01).Logistic多因素回归分析结果显示,血清IL-10水平为胃癌恶病质发生的独立危险因素(OR=1.710,95%CI为1.285～2.276).恶病质组患者IL-10基因-1082AG和-819CC基因型频率较非恶病质组患者均显著升高(P=0.036,P=0.024).Logistic多因素回归分析结果显示,-1082AG基因型的胃癌患者发生恶病质的风险较-1082AA基因型者增加(OR=1.989,95%CI为1.041～3.802),-819CC基因型的胃癌患者发生恶病质的风险较-819TT基因型者增加(OR=3.393,95%CI为1.298～8.871).单体型分析显示,IL-10基因GCC单体型为胃癌患者发生恶病质的高危因素(OR=2.21,95%CI为1.14～4.30).结论 IL-10基因单体型与胃癌患者恶病质的发生有关.

Abstract：

Objective To investigate whether the single nucleofide polymorphisms (SNPs) at -1082, -819 and -592 of interleukin-10 gene and its haplotype are associated with cachexia in patients with gastric cancer. Methods Radioimmunoassay was used to examine the serum levels of IL-10 in 223 patients with gastric cancer. The single nucleotide polymorphisms (SNPs) of IL-10 gene -1082G/A,-819T/C and -592A/C were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The serum levels of IL-10 were significantly higher in patients with cachexia than those without (P ＜0.001 ). An increased frequency of - 1082G allele was noted in patients with cachexia (P = 0. 049). The frequencies of -1082AG and -819CC genotypes were elevated in patients with cachexia than those without (P = 0.036, 0.024). In a logistic regression analysis adjusted for actual weight, carcinoma location and stage, the - 1082AG genotype was associated with an odds ratio of 1. 989 (95%CI, 1.041-3.802, P=0.037), and the -819CC genotype with an odds ratio of 3.393 (95% CI,1. 298-8.871, P= 0. 013 ) for cachexia. Furthermore, haplotype analysis revealed that G1082C819C592 haplotype was associated with a significantly increased risk of cachexia ( OR = 2.21; 95% CI, 1.14-4. 30;P =0.02). Conclusion Our results suggest that the gene haplotype of IL-10 contributes to the occurrence of cachexia in patients with gastric cancer in Chinese population.

Association of interlenkin-10 gene polymorphism with cachexia in patients with gastric cancer

Objective To investigate whether the single nucleofide polymorphisms (SNPs) at -1082, -819 and -592 of interleukin-10 gene and its haplotype are associated with cachexia in patients with gastric cancer. Methods Radioimmunoassay was used to examine the serum levels of IL-10 in 223 patients with gastric cancer. The single nucleotide polymorphisms (SNPs) of IL-10 gene -1082G/A,-819T/C and -592A/C were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The serum levels of IL-10 were significantly higher in patients with cachexia than those without (P ＜0.001 ). An increased frequency of - 1082G allele was noted in patients with cachexia (P = 0. 049). The frequencies of -1082AG and -819CC genotypes were elevated in patients with cachexia than those without (P = 0.036, 0.024). In a logistic regression analysis adjusted for actual weight, carcinoma location and stage, the - 1082AG genotype was associated with an odds ratio of 1. 989 (95%CI, 1.041-3.802, P=0.037), and the -819CC genotype with an odds ratio of 3.393 (95% CI,1. 298-8.871, P= 0. 013 ) for cachexia. Furthermore, haplotype analysis revealed that G1082C819C592 haplotype was associated with a significantly increased risk of cachexia ( OR = 2.21; 95% CI, 1.14-4. 30;P =0.02). Conclusion Our results suggest that the gene haplotype of IL-10 contributes to the occurrence of cachexia in patients with gastric cancer in Chinese population.