健脾增免中药对小鼠免疫机能及力竭性游泳运动能力的影响

目的 :观察健脾增免中药对小鼠免疫机能、血睾、血红蛋白、力竭性游泳运动能力的影响。方法 :采用3 H -TdR掺入法、ELISA法 ,12 5I-睾酮测定等。结果 :服用健脾增免中药 4周后 ,高剂量组 (ISSIH)IL-2水平显著高于其它各组 (P <0 0 0 1 ) ,脾细胞数明显高于对照组 (CON) (P <0 0 5 ) ,淋巴细胞对刀豆蛋白 (ConA)的增殖反应显著高于对照组 (P <0 0 1 )及阳性药物组 (PD) (P<0 0 5 ) ;中剂量组 (ISSIM)的淋巴细胞对脂多糖 (LPS)的增殖反应显著高于其它各组 (P <0 0 1 ) ;PD组血清睾酮 (T)显著高于其它各组 (P <0 0 0 1 ) ,ISSIH组T明显高于CON组 (P <0 0 5 )。该中药对体重 (W)、血红蛋白(Hb)无明显影响 ,但可明显延长小鼠力竭性游泳时间。毒性实验表明该药物安全无毒。结论 :健脾增免中药有增强机体免疫力、提高血清睾酮水平和抗疲劳的作用

Effects of Chinese Drugs for Invigorating Spleen and Strengthening Immunity on Immune Function and Exhaustive Swimming Capacity in Mice

Objective To study the effect of Chinese drugs for invigorating spleen and strengthening immunity (ISSI) on immune function and exhaustive swimming capacity in mice. Methods 3H-TdR, ELISA, 125 I-T (testosterone) and exhaustive swimming were used in the experiment. Results IL-2 of the high dose ISSI drug group(ISSIH)was much higher than those of other groups (P< 0 001) 4 weeks after taking ISSI; Lymphocyte response to ConA of ISSIH was significantly higher than that of control group (CON) (P< 0 01) and positive drugs group (PD) (P < 0 05); The number of cells of the spleen of ISSIH were significantly higher than that of CON (P < 0.05); Lymphocyte response to LPS of the middle dose ISSI drug group (ISSIM) was significantly higher than those of other groups (P< 0 01); T of the positive drug group (PD) was much higher than those of other groups (P< 0 001), T of ISSIH was significantly higher than that of CON (P< 0 05). ISSI drug has no significantly effects on weight and Hb, but it can lengthen the duration of exhaustive swimming. No toxicity was found in the drug. Conclusion ISSI drug could improve immunocompetence, testosterone, anti-fatigue and prolong the time of exhaustive swimming in mice.