| 腺病毒介导的BMP-2基因转染兔骨髓基质干细胞及对其增殖、成骨分化的影响 |
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| 引用本文： | 崔少千,范广宇,李建军,周凤华,于洪德,张磊,李雷,王欢. 腺病毒介导的BMP-2基因转染兔骨髓基质干细胞及对其增殖、成骨分化的影响[J]. 东南大学学报(医学版), 2009, 28(1) |
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| 作者姓名： | 崔少千 范广宇 李建军 周凤华 于洪德 张磊 李雷 王欢 |
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| 作者单位： | 1. 中国医科大学附属盛京医院,脊柱创伤外科,辽宁,沈阳,110004
2. 中国医科大学附属第一医院,骨科,辽宁,沈阳,110001 |
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| 摘 要： | 目的: 用骨形态发生蛋白-2(BMP-2)腺病毒表达载体(Ad-BMP-2)转染兔骨髓基质干细胞(BMSCs),研究转染对其增殖、成骨分化的影响.方法: 用Ad-BMP-2转染兔BMSCs,利用免疫细胞化学法、原位杂交染色和蛋白印迹法检测转染后细胞BMP-2的表达.流式细胞仪观察细胞周期的变化,分析转染对BMSCs增殖的影响.酶标法检测ALP活性;免疫荧光检测骨钙素(OCN)、Ⅰ型胶原表达,分析转染对BMSCs成骨分化的影响.结果: BMP-2基因在转染细胞内mRNA水平和蛋白水平均有较高表达,蛋白印迹法检测到培养液中有BMP-2蛋白阳性表达.流式细胞仪检测G1期、S期细胞比例与空白对照组无差异.ALP活性明显增高,骨钙素、Ⅰ型胶原免疫荧光检测,见胞浆内有大量显绿色荧光的阳性物质.结论: 在腺病毒载体介导下BMP-2基因成功导入BMSCs,细胞增殖未因转染而受影响,并可持续表达基因产物,向成骨细胞分化.
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| 关 键 词： | 腺病毒 骨形态发生蛋白-2 骨髓基质干细胞 基因治疗 |
Effects of adenovirus-BMP-2 transfection on proliferation and differentiation of rabit bone marrow stromal cells in vitro |
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| CUI Shao-qian,FAN Guang-yv,LI Jian-jun,ZHOU Feng-hua,YU Hong-de,ZHANG Lei,LI Lei,Wang Huan. Effects of adenovirus-BMP-2 transfection on proliferation and differentiation of rabit bone marrow stromal cells in vitro[J]. Journal of Southeast Univ: Medical Sci Ed, 2009, 28(1) |
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| Authors: | CUI Shao-qian FAN Guang-yv LI Jian-jun ZHOU Feng-hua YU Hong-de ZHANG Lei LI Lei Wang Huan |
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| Affiliation: | 1.Department of Orthopedics;Shengjing Hospital;China Medical University;Shenyang 110004;China;2.Department of Orthopedics;the First Affiliated Hospital;Shenyang 110001;China |
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| Abstract: | Objective To study the effects of adenovirus-BMP-2(Ad-BMP-2) transfection on proliferation and differentiation of rabit bone marrow stromal cells(BMSCs) in vitro.Methods Ad-BMP-2 transfected rabbit BMSCs.mRNA and protein expression of BMSCs were detected by BMP-2 hybridization in situ and immunocytochemical technique and Western blotting analysis.The change of BMSCs cell cycle after transfection was observed by flow cytometry.Alkaline phosphatase activity and staining,immunofluorescence analysis of osteocal... |
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| Keywords: | adenovirus bone morphogenetic protein-2 bone marrow stromal cells gene therapy |
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