不同方法检测新疆和田地区HBsAg弱阳性标本结果分析和应用评价

目的探讨新疆和田地区低水平HBsAg的检测方法及临床意义。方法样本为和田地区维吾尔医医院检验科经上海科华公司生产的HBsAg酶联免疫吸附试验（ELISA）试剂盒或雅培ARCHITECT i2000SR免疫发光分析仪初筛为弱阳性的标本。用丽珠乙型肝炎病毒表面抗原中和试剂盒（酶联免疫法）进行检测，并结合相应样本的肝功能及HBV—DNA检测结果进行分析。结果经科华试剂盒初筛为弱阳性的35例样本中，丽珠乙型肝炎病毒表面抗原中和试剂盒检测为阳性27例、阴性8例，此35例样本经化学发光法检测均为阳性；经化学发光法检测为弱阳性的10例样本中，丽珠乙型肝炎病毒表面抗原中和试剂盒检测为阳性4例，阴性6例。结论不同检测系统检测低水平HBsAg的结果存在差异。低水平HBsAg的临床价值有待进一步研究。

Results Analysis and Application Evaluation of Weekly Positive HBsAg Specimens Detected by Different Methods in Hetian of Xinjiang Uygur Autonomous Region

Objective To explore the detection method of low level HBsAg in serum and its clinical value in Hetian of Xinjiang Uygur Autonomous Region. Methods Serum samples were enzyme - linked immunosorbent assay （ELISA） kit from Kehua Company in Shanghai or weakly positive specimens screened by ABBOTT ARCHITECT i2000SR immunoluminous analyzer. Lizhu HBsAg neutralization kit（ELISA） was used in the detection. The results were analyzed with liver function and HBV - DNA test. Results Among 35 weakly positive specimens screened by Kehua kit,27 specimens were positive and 8 were negative tested by Lizhu HBsAg neutralization kit, while all the 35 specimens were positive tested by chemiluminescence. Among 10 weakly positive specimens by chemiluminescence,4 were positive and 6 were negative tested by Lizhu HBsAg neutralization kit. Conclusion The test results of low level HBsAg vary with detection systems. The clinical value of low level HBsAg need to be studied further.