| Bmi-1基因RNA干扰对胃癌SGC-7901细胞增殖和凋亡的影响 |
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| 引用本文： | 魏伟,王智,汪玲,肖军.Bmi-1基因RNA干扰对胃癌SGC-7901细胞增殖和凋亡的影响[J].医学新知杂志,2013(6):410-413,F0004. |
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| 作者姓名： | 魏伟 王智 汪玲 肖军 |
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| 作者单位： | [1]大悟县人民医院内四科,湖北大悟432800 [2]武汉大学中南医院消化内科,湖北武汉430071 |
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| 摘 要： | 目的构建针对Bmi-1基因的RNAi表达载体，观察其对胃癌细胞SGC-7901增殖和凋亡的影响。方法利用慢病毒表达体系pHelperl．0／pHelper2．0／pGCL—GFP，成功构建针对Bmi-1基因的RNAi重组质粒vshRNA—Bmi-1，适时定量PCR（real—timePCR）检测稳定转染后胃癌细胞SGC-7901中Bmi-1mRNA表达，流式细胞术检测细胞凋亡，MTr法检测其增殖能力。结果稳定转染vshRNA—Bmi-1后SGC-7901细胞Bmi-1mRNA表达明显下降，总抑制效率达85％；癌细胞增殖减慢，凋亡率明显增加。结论构建针对Bmi-1基因的RNAi表达载体，成功转染胃癌细胞SGC-7901，可促进癌细胞凋亡，抑制其增殖能力。
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| 关 键 词： | Bmi-1 RNAi 胃癌 凋亡 增殖 |
Effect of Bmi - 1 gene RNAi on Proliferation and Apoptosis of Gastric Carcinoma SGC- 7901 Cell |
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| Institution: | Wei Wei, Wang Zhi, Wang Ling, et al. (Forth Department of Internal Medicine, Dawu People＇s; Hospital, Dawu 432800,China ) |
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| Abstract: | Objective To construct Bmi - 1 RNAi expression vector and investigate its influence on proliferation and apoptosis of gastric carcinoma SGC - 7901 cells. Methods Recombinant plasmid vshRNA - Bmi - 1 was constructed by antiviral expression system pHelperl. 0/pHelper2.0/pGCL - GFP. Bmi - 1 mRNA expression in stably transfected gastric carcinoma cells was identified by real - time PCR. Cell apoptosis was detected by flow cytometry and cell proliferation was detected by MTF. Results Bmi - 1 mRNA expression in stably transfected SGC -7901 cells was significantly decreased with total inhibition efficiency of 85%. The proliferation of carcinoma cells slowed down and apoptosis rate increased significantly. Conclusion Successful construction of Bmi - 1 RNAi expression vector with transfected SGC - 7901 cells can promote apoptosis of gastric carcinoma cells and suppress proliferation of them. |
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| Keywords: | Bmi - 1 RNAi Gastric carcinoma Apoptosis Proliferation |
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