HLA-DRB1*1501位点基因多态性与重度慢性牙周炎易感性的相关关系研究

目的探讨HLA-Ⅱ类基因区基因多态性与重度慢性牙周炎易感性的关系.方法收集134例重度慢性牙周炎(CP)患者和81例健康对照者的颊黏膜拭子,提取DNA,采用多聚酶链反应-限制性内切酶片段长度多态性(PCR-RELP)方法,检测HLA-DRB1*150l的多态性,采用行×列表资料的x2检验,比较重度CP患者和健康对照组中等位基因频率和基因型的分布.结果DRB1*150l等位基因频率在重度CP组高于对照组(P＜0.05),0R=3.874(2.401～6.252),提示该等位基因可能与重度CP的危险性增高有关.两组之间基因型分布有统计学差异,携带HLADRB1*1501纯合子基因型个体患重度CP的危险性可能增高,0R=20.896 (4.866～89.726),P＜0.05.结论DRB1*1501等位基因可能与汉族人群重度CP遗传易感性相关,HLA-DRB1*1501纯合子基因型可能增加了重度CP的易感性.

Association of HLA-DRB1*1501 polymorphism with the susceptibility to severe chronic periodontitis in Chinese Han nationality

PURPOSE: To investigate the association of HLA-DRB1*1501 polymorphism with the susceptibility to severe chronic periodontitis (CP) in Chinese Han nationality. METHODS: DNA samples were collected with buccal swabs from 134 subjects with severe chronic periodontitis and 81 periodontal healthy control. HLA-DRB1*1501 genotype was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype distribution was statistically analyzed by chi2 test. RESULTS: The allele frequencies of DRB1*1501 was detected more frequently in severe CP patients than in the controls [OR=3.874 (2.401-6.252), P<0.05]. The 1501/1501 genotype frequencies were significantly increased in patients compared with the reference population [OR=20.896 (4.866-89.726), P<0.05]. CONCLUSION: The results suggest that HLA-DRB1*1501 allele may be a risk indicator for the susceptibility to severe CP in Han nationality (P<0.05). The HLA-DRB1*1501 homozygote genotype was associated significantly with severe CP (P<0.05).