扶正胶囊中人参皂苷Rg_1与Re及Rb_1的质量测定

目的:建立扶正胶囊中人参皂苷Rg1、Re、Rb1超高效液相色谱(UPLC)测定方法。方法:采用Acquity1UPLCBEHC18色谱柱(2.1mm×100mm,1.7μm),以乙腈-水为流动相进行梯度洗脱,流速0.5mL/min,检测波长203nm,柱温40℃。结果:扶正胶囊中人参皂苷Rg1、Re、Rb1在14min内完全分离;人参皂苷Rg1和人参皂苷Re分离度达2.0,人参皂苷Rg1、Re、Rb1峰理论板数均超过20000;经外标法计算,含人参皂苷Rg1、Re、Rb1质量分别为0.71mg/g,3.841mg/g,10.61mg/g。结论:本法简便、准确、快速,可用于扶正胶囊中人参皂苷Rg1、Re、Rb1含质量测定。

Determination of Ginsenoside-Rg1 & Re & Rb1 in Fuzheng Capsule by UPLC

Objective：To establish the determination of ginsenoside-Rg1,Re and Rb1 in Fuzheng capsule by ultra high performance liquid chromatography（UPLC）.Methods：Using Acquity UPLC BEH C18（2.1 mm×100 mm,1.7μm）,the column temperature set at 40 ℃,the mobile phase was composed of acetonitrile and water by gradient elution with a flow rate at 0.5 mL/min,and the range of wavelength was at 203 nm.Results：The Ginsenoside-Rg1,Re and Rb1 in Fuzheng capsule were seperated completely in 14 min,the efficiency of separation was 2.0,and the number of theoretical plate was more than 20 000.The quantitative determination of Ginsenoside-Rg1,Re and Rb1 in Fuzheng capsule could be done by external standard method and it was 0.71,3.84,10.61 mg/g respectively.Conclusion：The method was rapid,sensitive and accurate.It can be used for quality control of ginsenoside-Rg1,Re and Rb1 in Fuzheng capsule.