姜黄素对丙烯酰胺致HepG2细胞DNA损伤的保护作用

目的体外研究姜黄素对丙烯酰胺(AA)所致人肝癌HepG2细胞DNA损伤的保护作用。方法AA(0、2.5、5.0、10.0和20.0mmol/L)与HepG2细胞温育1h,单细胞凝胶电泳检测DNA损伤;姜黄素(0.63、1.25和2.50μg/ml)37℃预处理HepG2细胞1h后,再与不同浓度的AA(0、10和20mmol/L)温育1h,单细胞凝胶电泳检测DNA损伤,2',7'-二氢二氯荧光比色法测定细胞内活性氧水平。结果2.5、5.0、10.0和20.0mmol/LAA组的彗尾DNA含量、彗星尾长及彗星尾矩均显著高于对照组(P<0.05),姜黄素保护组的彗尾DNA含量、彗星尾长及彗星尾矩均比相同浓度AA处理组降低,2.50μg/ml姜黄素保护组的差异有显著性(P<0.05);AA(10和20mmol/L)作用于HepG2细胞1h后,细胞内ROS水平显著升高(P<0.01),2.50μg/ml姜黄素预处理组ROS水平显著低于单独AA处理组(P<0.01)。结论姜黄素对AA所致HepG2细胞DNA损伤具有保护作用。

Preventive effects of curcumin on acrylamide-induced DNA damage in HepG2 cells

Objectlve To investigate the protective effects of curcumin on acrylamide (AA)-induced DNA damage in HepG2 cells. Methods After pretreatment with eurcumin (0.63,1.25 and 2.50μg/ml) for l hour, HepG2 cells were exposed to various concentrations of AA (0, 10, 20mmol/L) for 1h, comet assay was performed to determine DNA damage. The production of reactive oxygen species (ROS) was measured using the 2, 7-dichlorofluorescein diacetate (DCFH-DA) method. Results One-hour exposure of HepG2 cells to AA led to a dose-dependent increase in DNA fragmentation. When the cells were pretreated with curcumin for 1 h, the comet tail moment values were decreased compared to only AA-treated cells, and curoumin of 2.50μg/ml significantly decreased the comet tail moment values compared to only AA-treated cells (P < 0.05). The DCF fluorescence intensity increased when the HepG2 cells were treated with 10 and 20 mmol/L AA ( P < 0.01). When the cells were pretreated with cureumin, the level of ROS was significantly decreased compared to only AA-treated ( P < 0.01 ). Conclusion These data suggest that eurcumin could attenuate AA-induced DNA damage in HepG2 cells. The protection is probably mediated by antioxidant protective mechanism.