| A method for mouse oocyte enucleation assisted with zona pellucida dilating |
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| 作者姓名： | 白照岱 刘凯 邴鲁军 |
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| 作者单位： | Department of Histology and Embryology，Medical College of Shandong University，Jinan 250012，China，Department of Histology and Embryology，Medical College of Shandong University，Jinan 250012，China，Department of Histology and Embryology，Medical College of Shandong University，Jinan 250012，China |
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| 摘 要： | <正>Objective: To study the reliability of a new enucleation method, zona pellucida Dilating (ZPD) assisted enucleation method, for mouse oocyte enucleation. Methods: In ZPD enucleation method, the zona pellucida was dilating with the help of outside pression in enucleation needle after breakthrough by needle tip and the nucleus was aspirated by the needle entering the perivitelline space for enucleation. This method was employed and was compared with three other methods in this experiment. The efficiency of enucleation of mouse oocyte was examed. Result:ZPD assisted enucleation method is better than three others at the survival rate after enucleation and the simplicity of micromanipulation. The rate of forming pronucleus of reconstructed embryo from enucleated oocyte by ZPD methods is as high as 62. 8% and the reconstructed embryo at 4-cell stage was obtained. Conclusion: ZPD assisted method is a highly efficient and simple enucleation method with the advantage of saving manipulating time and it does less damage to the oocyte.
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A method for mouse oocyte enucleation assisted with zona pellucida dilating |
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| BAI Zhao-dai,Liu Kai,BING Lu-Jun.A method for mouse oocyte enucleation assisted with zona pellucida dilating[J].Journal of Reproductive Medicine,2004,13(Z1). |
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| Authors: | BAI Zhao-dai Liu Kai BING Lu-Jun |
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| Institution: | Department of Histology and Embryology, Medical College of Shandong University, Jinan 250012, China |
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| Abstract: | Objective: To study the reliability of a new enucleation method, zona pellucida Dilating (ZPD) assisted enucleation method, for mouse oocyte enucleation. Methods: In ZPD enucleation method, the zona pellucida was dilating with the help of outside pression in enucleation needle after breakthrough by needle tip and the nucleus was aspirated by the needle entering the perivitelline space for enucleation. This method was employed and was compared with three other methods in this experiment. The efficiency of enucleation of mouse oocyte was examed. Result:ZPD assisted enucleation method is better than three others at the survival rate after enucleation and the simplicity of micromanipulation. The rate of forming pronucleus of reconstructed embryo from enucleated oocyte by ZPD methods is as high as 62. 8% and the reconstructed embryo at 4-cell stage was obtained. Conclusion: ZPD assisted method is a highly efficient and simple enucleation method with the advantage of saving manipulating time and it does less damage to the oocyte. |
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| Keywords: | Zona pellucida Mouse Enucleation Oocyte |
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