检测支气管肺泡灌洗液中的肿瘤标志物和miR-29a、miR-34a对肺癌诊断和分型的作用

目的探讨检测支气管肺泡灌洗液（BALF）中的CEA、CA125、NSE、CyFRA21—1肿瘤标志物和miR-29a、miR-34a对肺癌诊断和分型的作用。方法收集105例疑似肺癌患者BALF，分为肺部良性疾病组（c组）25例、小细胞肺癌组（SCLC组）22例、鳞癌组（SQ组）34例、腺癌组（AC组）24例，分别检测各组CEA、CA125、NSE、Cy．FRA21-1和miR-29a、miR-34a的水平，并对各组的数据进行统计学处理和比较。结果SCLC组、SQ组、AC组3组的BALF中的CEA、CA125、NSE、CyFRA21—1水平均显著高于肺部良性疾病组（P〈0．05），而SCLC组、sQ组、AC组3组之间差异无统计学意义（P〉0．05）；SCLC组BALF中的miR-29a表达较sQ和AC两组显著下调（P〈0．05），而SQ和AC两组之间差异无统计学意义（P〉0．05）；AC组BALF中的miR-34a表达较SCLC和SQ两组显著下调（P〈0．05），而SCLC和sQ两组之间差异无统计学意义（P〉0．05）。结论检测BALF中的肿瘤标志物和miR-29a、miR-34a对肺癌的诊断和分型具有重要的临床意义。

Effects of detecting tumor markers,mir-29a and mir-34a in balf for lung cancer diagnosis and classification

Objective To investigate the effects of detecting tumor markers （ CEA, CA125, NSE, CyFRA21-1 ）, miR-29a and miR-34a in BALF for lung cancer diagnosis and classification. Methods To analyze the levels of CEA, CA125, NSE,CyFRA21-1 and miR-29a,miR-34a in BALF of 105 suspected patients with lung cancer, the patients were divided into four groups （25 patients with benign lung disease, 22 with SCLC, 34 with SQ and 24 with AC） ;the data of these groups were analyzed statistically and compared. Results Compared with the benign lung disease group, the levels of CEA, CA125, NSE, CyFRA21-1 in BALF of patients in SCLC, SQ and AC group were significantly increased（ P 〈 0.05 ）, while there were no significant differences in SCLC,SQ and AC group. Compared with the SQ group and the AC group, the levels of miR-29a in BALF of the SCLC group were significantly decreased （P 〈 0.05 ）, while there were no signifi- cant differences between the SQ group and the AC group. Compared with the SCLC group and the SQ group, the levels of miR-34a in BALF of the AC group were significantly decreased （ P 〈 0.05 ）, while there were no significant differences between the SCLC group and the SQ group. Conclusion The levels of tumor markers, miR-29a and miR-34a in BALF had a great clinical value for lung cancer diagnosis and classification.